caa a22 4500 606211845 CHVBK 20210128101040.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10295-014-1544-y doi (NATIONALLICENCE)springer-10.1007/s10295-014-1544-y Assessing the potential of wild yeasts for bioethanol production [Elektronische Daten] [Stefan Ruyters, Vaskar Mukherjee, Kevin Verstrepen, Johan Thevelein, Kris Willems, Bart Lievens] Bioethanol fermentations expose yeasts to a new, complex and challenging fermentation medium with specific inhibitors and sugar mixtures depending on the type of carbon source. It is, therefore, suggested that the natural diversity of yeasts should be further exploited in order to find yeasts with good ethanol yield in stressed fermentation media. In this study, we screened more than 50 yeast isolates of which we selected five isolates with promising features. The species Candida bombi, Wickerhamomyces anomalus and Torulaspora delbrueckii showed better osmo- and hydroxymethylfurfural tolerance than Saccharomyces cerevisiae. However, S. cerevisiae isolates had the highest ethanol yield in fermentation experiments mimicking high gravity fermentations (25% glucose) and artificial lignocellulose hydrolysates (with a myriad of inhibitors). Interestingly, among two tested S. cerevisiae strains, a wild strain isolated from an oak tree performed better than Ethanol Red, a S. cerevisiae strain which is currently commonly used in industrial bioethanol fermentations. Additionally, a W. anomalus strain isolated from sugar beet thick juice was found to have a comparable ethanol yield, but needed longer fermentation time. Other non-Saccharomyces yeasts yielded lower ethanol amounts. Society for Industrial Microbiology and Biotechnology, 2014 Bioethanol nationallicence Lignocellulose nationallicence Nectar nationallicence Wickerhamomyces anomalus nationallicence Thick juice nationallicence Ruyters Stefan Laboratory for Process Microbial Ecology and Bioinspirational Management, Department of Microbial and Molecular Systems (M2S), Cluster for Bioengineering Technology (CBeT), KU Leuven, Campus De Nayer, Fortsesteenweg 30A, 2860, Sint-Katelijne-Waver, Belgium aut Mukherjee Vaskar Laboratory for Process Microbial Ecology and Bioinspirational Management, Department of Microbial and Molecular Systems (M2S), Cluster for Bioengineering Technology (CBeT), KU Leuven, Campus De Nayer, Fortsesteenweg 30A, 2860, Sint-Katelijne-Waver, Belgium aut Verstrepen Kevin Laboratory for Genetics and Genomics, VIB Laboratory for Systems Biology, Centre of Microbial and Plant Genetics (CMPG), M2S, KU Leuven, Gaston Geenslaan 1, 3001, Leuven, Belgium aut Thevelein Johan Laboratory of Molecular Cell Biology, VIB Department of Molecular Microbiology, Institute of Botany and Microbiology, KU Leuven, Kasteelpark Arenberg 31, 3001, Leuven, Belgium aut Willems Kris Laboratory for Process Microbial Ecology and Bioinspirational Management, Department of Microbial and Molecular Systems (M2S), Cluster for Bioengineering Technology (CBeT), KU Leuven, Campus De Nayer, Fortsesteenweg 30A, 2860, Sint-Katelijne-Waver, Belgium aut Lievens Bart Laboratory for Process Microbial Ecology and Bioinspirational Management, Department of Microbial and Molecular Systems (M2S), Cluster for Bioengineering Technology (CBeT), KU Leuven, Campus De Nayer, Fortsesteenweg 30A, 2860, Sint-Katelijne-Waver, Belgium aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 39-48 1367-5435 42:1<39 2015 42 10295 https://doi.org/10.1007/s10295-014-1544-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1544-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ruyters Stefan Laboratory for Process Microbial Ecology and Bioinspirational Management, Department of Microbial and Molecular Systems (M2S), Cluster for Bioengineering Technology (CBeT), KU Leuven, Campus De Nayer, Fortsesteenweg 30A, 2860, Sint-Katelijne-Waver, Belgium aut NATIONALLICENCE 700 1- Mukherjee Vaskar Laboratory for Process Microbial Ecology and Bioinspirational Management, Department of Microbial and Molecular Systems (M2S), Cluster for Bioengineering Technology (CBeT), KU Leuven, Campus De Nayer, Fortsesteenweg 30A, 2860, Sint-Katelijne-Waver, Belgium aut NATIONALLICENCE 700 1- Verstrepen Kevin Laboratory for Genetics and Genomics, VIB Laboratory for Systems Biology, Centre of Microbial and Plant Genetics (CMPG), M2S, KU Leuven, Gaston Geenslaan 1, 3001, Leuven, Belgium aut NATIONALLICENCE 700 1- Thevelein Johan Laboratory of Molecular Cell Biology, VIB Department of Molecular Microbiology, Institute of Botany and Microbiology, KU Leuven, Kasteelpark Arenberg 31, 3001, Leuven, Belgium aut NATIONALLICENCE 700 1- Willems Kris Laboratory for Process Microbial Ecology and Bioinspirational Management, Department of Microbial and Molecular Systems (M2S), Cluster for Bioengineering Technology (CBeT), KU Leuven, Campus De Nayer, Fortsesteenweg 30A, 2860, Sint-Katelijne-Waver, Belgium aut NATIONALLICENCE 700 1- Lievens Bart Laboratory for Process Microbial Ecology and Bioinspirational Management, Department of Microbial and Molecular Systems (M2S), Cluster for Bioengineering Technology (CBeT), KU Leuven, Campus De Nayer, Fortsesteenweg 30A, 2860, Sint-Katelijne-Waver, Belgium aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 39-48 1367-5435 42:1<39 2015 42 10295