caa a22 4500 60621187X CHVBK 20210128101040.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10295-014-1533-1 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1533-1 Microbial diversity in sugarcane ethanol production in a Brazilian distillery using a culture-independent method [Elektronische Daten] [Ohana Costa, Betulia Souto, Daiva Tupinambá, Jessica Bergmann, Cynthia Kyaw, Ricardo Kruger, Cristine Barreto, Betania Quirino] Sugarcane ethanol production occurs in non-sterile conditions, and microbial contamination can decrease productivity. In this study, we assessed the microbial diversity of contaminants of ethanol production in an industrial facility in Brazil. Samples obtained at different stages were analyzed by pyrosequencing-based profiling of bacterial and archaeal 16S rRNA genes and the fungal internal transcribed spacer region. A total of 355 bacterial groups, 22 archaeal groups, and 203 fungal groups were identified, and community changes were related to temperature changes at certain stages. After fermentation, Lactobacillus and unclassified Lactobacillaceae accounted for nearly 100% of the bacterial sequences. Predominant Fungi groups were "unclassified Fungi,” Meyerozyma, and Candida. The predominant Archaea group was unclassified Thaumarchaeota. This is the first work to assess the diversity of Bacteria, and Archaea and Fungi associated with the industrial process of sugarcane-ethanol production using culture-independent techniques. Society for Industrial Microbiology and Biotechnology, 2014 Ethanol nationallicence Fermentation nationallicence Biodiversity nationallicence Industry nationallicence Microbiology nationallicence Costa Ohana Genomic Sciences and Biotechnology Program, Universidade Católica de Brasília, 70790-160, Brasília, DF, Brazil aut Souto Betulia Embrapa-Agroenergy, Parque Estação Biológica, s/n, 70770-901, Brasília, DF, Brazil aut Tupinambá Daiva Genomic Sciences and Biotechnology Program, Universidade Católica de Brasília, 70790-160, Brasília, DF, Brazil aut Bergmann Jessica Genomic Sciences and Biotechnology Program, Universidade Católica de Brasília, 70790-160, Brasília, DF, Brazil aut Kyaw Cynthia Department of Cellular Biology, Universidade de Brasilia, 70910-900, Brasília, DF, Brazil aut Kruger Ricardo Department of Cellular Biology, Universidade de Brasilia, 70910-900, Brasília, DF, Brazil aut Barreto Cristine Genomic Sciences and Biotechnology Program, Universidade Católica de Brasília, 70790-160, Brasília, DF, Brazil aut Quirino Betania Genomic Sciences and Biotechnology Program, Universidade Católica de Brasília, 70790-160, Brasília, DF, Brazil aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 73-84 1367-5435 42:1<73 2015 42 10295 https://doi.org/10.1007/s10295-014-1533-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1533-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Costa Ohana Genomic Sciences and Biotechnology Program, Universidade Católica de Brasília, 70790-160, Brasília, DF, Brazil aut NATIONALLICENCE 700 1- Souto Betulia Embrapa-Agroenergy, Parque Estação Biológica, s/n, 70770-901, Brasília, DF, Brazil aut NATIONALLICENCE 700 1- Tupinambá Daiva Genomic Sciences and Biotechnology Program, Universidade Católica de Brasília, 70790-160, Brasília, DF, Brazil aut NATIONALLICENCE 700 1- Bergmann Jessica Genomic Sciences and Biotechnology Program, Universidade Católica de Brasília, 70790-160, Brasília, DF, Brazil aut NATIONALLICENCE 700 1- Kyaw Cynthia Department of Cellular Biology, Universidade de Brasilia, 70910-900, Brasília, DF, Brazil aut NATIONALLICENCE 700 1- Kruger Ricardo Department of Cellular Biology, Universidade de Brasilia, 70910-900, Brasília, DF, Brazil aut NATIONALLICENCE 700 1- Barreto Cristine Genomic Sciences and Biotechnology Program, Universidade Católica de Brasília, 70790-160, Brasília, DF, Brazil aut NATIONALLICENCE 700 1- Quirino Betania Genomic Sciences and Biotechnology Program, Universidade Católica de Brasília, 70790-160, Brasília, DF, Brazil aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 73-84 1367-5435 42:1<73 2015 42 10295