caa a22 4500 606211888 CHVBK 20210128101040.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10295-014-1527-z doi (NATIONALLICENCE)springer-10.1007/s10295-014-1527-z Overexpression of specific proton motive force-dependent transporters facilitate the export of surfactin in Bacillus subtilis [Elektronische Daten] [Xu Li, Huan Yang, Donglai Zhang, Xue Li, Huimin Yu, Zhongyao Shen] A novel surfactin producer, Bacillus subtilis THY-7, was isolated from soil. Using liposomes and transmembrane transport inhibitors, the surfactin efflux in THY-7 was determined to be mainly dependent on proton motive force (PMF), not ATP hydrolysis. YcxA, KrsE and YerP, three putative lipopeptide transporters with PMF as energy source, were then highlighted in this work. A mutant YcxA named as YcxAmt, with 2 transmembrane helices deletion due to a code-shift mutation of the encoding gene, was identified in THY-7. This truncated YcxAmt was confirmed unable to transfer surfactin; on the contrary, overexpression of the natural full-lengthYcxA enhanced the secretion of surfactin by 89%. KrsE, a putative kurstakin transporter, was found also capable of transporting surfactin. Overexpression of KrsE increased the production of surfactin by 52%. In the culture of YerP-overexpressing strain at 24h, surfactin titer reached 1.58gL−1, which was 145% higher than that of the control. This indicated that YerP acted as the major surfactin exporter in B. subtilis THY-7. Society for Industrial Microbiology and Biotechnology, 2014 Bacillus subtilis nationallicence Surfactin nationallicence Proton motive force nationallicence Overexpression nationallicence Lipopeptide transporters nationallicence Li Xu Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, 100084, Beijing, China aut Yang Huan Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, 100084, Beijing, China aut Zhang Donglai Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, 100084, Beijing, China aut Li Xue Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, 100084, Beijing, China aut Yu Huimin Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, 100084, Beijing, China aut Shen Zhongyao Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, 100084, Beijing, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 93-103 1367-5435 42:1<93 2015 42 10295 https://doi.org/10.1007/s10295-014-1527-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1527-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Xu Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Yang Huan Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Zhang Donglai Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Li Xue Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Yu Huimin Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Shen Zhongyao Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 93-103 1367-5435 42:1<93 2015 42 10295