caa a22 4500 606211896 CHVBK 20210128101040.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10295-014-1532-2 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1532-2 Heterologous expression of hyperthermophilic cellulases of archaea Pyrococcus sp. by fungus Talaromyces cellulolyticus [Elektronische Daten] [Seiichiro Kishishita, Tatsuya Fujii, Kazuhiko Ishikawa] Talaromyces cellulolyticus (formerly known as Acremonium cellulolyticus) is one of the high cellulolytic enzyme-producing fungi. T. cellulolyticus exhibits the potential ability for high amount production of enzyme proteins. Using the homologous expression system under the control of a glucoamylase promoter, some kinds of cellulases of T. cellulolyticus can be expressed by T. cellulolyticus. On the other hand, hyperthermophilic cellulase has been expected to be useful in the industrial applications to biomass. The hyperthermophilic archaea Pyrococcus horikoshii and P. furiosus have GH family 5 and 12 hyperthermophilic endocellulase, respectively. The two kinds of hyperthermophilic endocellulases were successfully produced by T. cellulolyticus using the above expression system under the control of a glucoamylase promoter of T. cellulolyticus. These recombinant cellulases exhibited the same characteristics as those of the recombinant cellulases prepared in E. coli. The productions of the recombinant enzymes were estimated to be over 100mg/L. In this study, we first report the overexpression of the hyperthermophilic enzymes of archaea using the fungal expression system. The Author(s), 2014 Biomass nationallicence Cellulase nationallicence Fungus nationallicence Archaea nationallicence Production nationallicence Kishishita Seiichiro Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, 739-0046, Higashi-Hiroshima, Hiroshima, Japan aut Fujii Tatsuya Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, 739-0046, Higashi-Hiroshima, Hiroshima, Japan aut Ishikawa Kazuhiko Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, 739-0046, Higashi-Hiroshima, Hiroshima, Japan aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 137-141 1367-5435 42:1<137 2015 42 10295 https://doi.org/10.1007/s10295-014-1532-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1532-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kishishita Seiichiro Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, 739-0046, Higashi-Hiroshima, Hiroshima, Japan aut NATIONALLICENCE 700 1- Fujii Tatsuya Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, 739-0046, Higashi-Hiroshima, Hiroshima, Japan aut NATIONALLICENCE 700 1- Ishikawa Kazuhiko Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology, 3-11-32 Kagamiyama, 739-0046, Higashi-Hiroshima, Hiroshima, Japan aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 137-141 1367-5435 42:1<137 2015 42 10295