caa a22 4500 60621190X CHVBK 20210128101040.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10295-014-1551-z doi (NATIONALLICENCE)springer-10.1007/s10295-014-1551-z Production of antibacterial Bombyx mori cecropin A in mealworm-pathogenic Beauveria bassiana ERL1170 [Elektronische Daten] [Se Lee, Jeong Yu, Bruce Parker, Margaret Skinner, Yeon Je, Jae Kim] Efforts are underway to produce antimicrobial peptides in yellow mealworms (Tenebrio molitor), which can be developed as more effective and safer animal feed additives. In this work, we expressed Bombyx mori (Bm) cecropin-A in mealworms by the infection of transformed entomopathogenic Beauveria bassiana ERL1170. The active domain of Bm cecropin A gene was tagged with a signal sequence of B. bassiana for extracellular secretion, and the fragment was inserted into ERL1170 by the restriction enzyme-mediated integration method. Transformant D-6 showed antibacterial activity against Bacillus subtilis and Listeria monocytogenes. Against T. molitor larvae, D-6 had similar mortality to wild-type, and D6-infected mealworm suspension showed strong antibacterial activity against the two bacteria, but not in the wild-type-infected mealworms, thereby increasing the value of mealworms as animal feed additives. Society for Industrial Microbiology and Biotechnology, 2014 Tenebrio molitor nationallicence Animal feed nationallicence Cecropin A nationallicence Beauveria bassiana nationallicence Antibacterial activity nationallicence Lee Se Department of Agricultural Biology, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, Korea aut Yu Jeong Department of Agricultural Biology, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, Korea aut Parker Bruce Department of Agricultural Biology, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, Korea aut Skinner Margaret Entomology Research Laboratory, University of Vermont, Burlington, VT, USA aut Je Yeon Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea aut Kim Jae Department of Agricultural Biology, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, Korea aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 151-156 1367-5435 42:1<151 2015 42 10295 https://doi.org/10.1007/s10295-014-1551-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1551-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Se Department of Agricultural Biology, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, Korea aut NATIONALLICENCE 700 1- Yu Jeong Department of Agricultural Biology, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, Korea aut NATIONALLICENCE 700 1- Parker Bruce Department of Agricultural Biology, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, Korea aut NATIONALLICENCE 700 1- Skinner Margaret Entomology Research Laboratory, University of Vermont, Burlington, VT, USA aut NATIONALLICENCE 700 1- Je Yeon Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Jae Department of Agricultural Biology, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju, Korea aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 151-156 1367-5435 42:1<151 2015 42 10295