caa a22 4500 606211918 CHVBK 20210128101040.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10295-014-1529-x doi (NATIONALLICENCE)springer-10.1007/s10295-014-1529-x Exploring the diversity and metabolic potential of actinomycetes from temperate marine sediments from Newfoundland, Canada [Elektronische Daten] [K. Duncan, B. Haltli, K. Gill, H. Correa, F. Berrué, R. Kerr] Marine sediments from Newfoundland, Canada were explored for biotechnologically promising Actinobacteria using culture-independent and culture-dependent approaches. Culture-independent pyrosequencing analyses uncovered significant actinobacterial diversity (H′—2.45 to 3.76), although the taxonomic diversity of biotechnologically important actinomycetes could not be fully elucidated due to limited sampling depth. Assessment of culturable actinomycete diversity resulted in the isolation of 360 actinomycetes representing 59 operational taxonomic units, the majority of which (94%) were Streptomyces. The biotechnological potential of actinomycetes from NL sediments was assessed by bioactivity and metabolomics-based screening of 32 representative isolates. Bioactivity was exhibited by 41% of isolates, while 11% exhibited unique chemical signatures in metabolomics screening. Chemical analysis of two isolates resulted in the isolation of the cytotoxic metabolite 1-isopentadecanoyl-3β-d-glucopyranosyl-X-glycerol from Actinoalloteichus sp. 2L868 and sungsanpin from Streptomyces sp. 8LB7. These results demonstrate the potential for the discovery of novel bioactive metabolites from actinomycetes isolated from Atlantic Canadian marine sediments. Society for Industrial Microbiology and Biotechnology, 2014 Duncan K. Department of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, C1A 4P3, Charlottetown, PE, Canada aut Haltli B. Department of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, C1A 4P3, Charlottetown, PE, Canada aut Gill K. Department of Chemistry, University of Prince Edward Island, 550 University Avenue, C1A 4P3, Charlottetown, PE, Canada aut Correa H. Nautilus Biosciences Canada Inc, C1A 4P3, Charlottetown, PE, Canada aut Berrué F. Department of Chemistry, University of Prince Edward Island, 550 University Avenue, C1A 4P3, Charlottetown, PE, Canada aut Kerr R. Department of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, C1A 4P3, Charlottetown, PE, Canada aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 57-72 1367-5435 42:1<57 2015 42 10295 https://doi.org/10.1007/s10295-014-1529-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1529-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Duncan K. Department of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, C1A 4P3, Charlottetown, PE, Canada aut NATIONALLICENCE 700 1- Haltli B. Department of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, C1A 4P3, Charlottetown, PE, Canada aut NATIONALLICENCE 700 1- Gill K. Department of Chemistry, University of Prince Edward Island, 550 University Avenue, C1A 4P3, Charlottetown, PE, Canada aut NATIONALLICENCE 700 1- Correa H. Nautilus Biosciences Canada Inc, C1A 4P3, Charlottetown, PE, Canada aut NATIONALLICENCE 700 1- Berrué F. Department of Chemistry, University of Prince Edward Island, 550 University Avenue, C1A 4P3, Charlottetown, PE, Canada aut NATIONALLICENCE 700 1- Kerr R. Department of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, C1A 4P3, Charlottetown, PE, Canada aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 57-72 1367-5435 42:1<57 2015 42 10295