caa a22 4500 606211926 CHVBK 20210128101040.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10295-014-1535-z doi (NATIONALLICENCE)springer-10.1007/s10295-014-1535-z Genetic improvement of native xylose-fermenting yeasts for ethanol production [Elektronische Daten] [Nicole Harner, Xin Wen, Paramjit Bajwa, Glen Austin, Chi-Yip Ho, Marc Habash, Jack Trevors, Hung Lee] Lignocellulosic substrates are the largest source of fermentable sugars for bioconversion to fuel ethanol and other valuable compounds. To improve the economics of biomass conversion, it is essential that all sugars in potential hydrolysates be converted efficiently into the desired product(s). While hexoses are fermented into ethanol and some high-value chemicals, the bioconversion of pentoses in hydrolysates remains inefficient. This remains one of the key challenges in lignocellulosic biomass conversion. Native pentose-fermenting yeasts can ferment both glucose and xylose in lignocellulosic biomass to ethanol. However, they perform poorly in the presence of hydrolysate inhibitors, exhibit low ethanol tolerance and glucose repression, and ferment pentoses less efficiently than the main hexoses glucose and mannose. This paper reviews classical and molecular strain improvement strategies applied to native pentose-fermenting yeasts for improved ethanol production from xylose and lignocellulosic substrates. We focus on Pachysolen tannophilus, Scheffersomyces (Candida) shehatae, Scheffersomyces (Pichia) stipitis, and Spathaspora passalidarum which are good ethanol producers among the native xylose-fermenting yeasts. Strains obtained thus far are not robust enough for efficient ethanol production from lignocellulosic hydrolysates and can benefit from further improvements. Society for Industrial Microbiology and Biotechnology, 2014 Adaptation nationallicence Cofactor imbalance nationallicence Genome shuffling nationallicence Metabolic engineering nationallicence Protoplast fusion nationallicence Random mutagenesis nationallicence Xylose reductase nationallicence Harner Nicole School of Environmental Sciences, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Wen Xin School of Environmental Sciences, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Bajwa Paramjit School of Environmental Sciences, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Austin Glen BP Biofuels, 92121, San Diego, CA, USA aut Ho Chi-Yip Microarray Laboratory, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, M5G 1X5, Toronto, ON, Canada aut Habash Marc School of Environmental Sciences, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Trevors Jack School of Environmental Sciences, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Lee Hung School of Environmental Sciences, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 1-20 1367-5435 42:1<1 2015 42 10295 https://doi.org/10.1007/s10295-014-1535-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1535-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harner Nicole School of Environmental Sciences, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Wen Xin School of Environmental Sciences, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Bajwa Paramjit School of Environmental Sciences, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Austin Glen BP Biofuels, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Ho Chi-Yip Microarray Laboratory, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, M5G 1X5, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Habash Marc School of Environmental Sciences, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Trevors Jack School of Environmental Sciences, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Lee Hung School of Environmental Sciences, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 1-20 1367-5435 42:1<1 2015 42 10295