caa a22 4500 606211934 CHVBK 20210128101040.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10295-014-1543-z doi (NATIONALLICENCE)springer-10.1007/s10295-014-1543-z Improved conversion efficiencies for n -fatty acid reduction to primary alcohols by the solventogenic acetogen " Clostridium ragsdalei ” [Elektronische Daten] [Catherine Isom, Mark Nanny, Ralph Tanner] "Clostridium ragsdalei” is an acetogen that ferments synthesis gas (syngas, predominantly H2:CO2:CO) to ethanol, acetate, and cell mass. Previous research showed that C. ragsdalei could also convert propionic acid to 1-propanol and butyric acid to 1-butanol at conversion efficiencies of 72.3 and 21.0 percent, respectively. Our research showed that C. ragsdalei can also reduce pentanoic and hexanoic acid to the corresponding primary alcohols. This reduction occurred independently of growth in an optimized medium with headspace gas exchange (vented and gassed with CO) every 48h. Under these conditions, conversion efficiencies increased to 97 and 100% for propionic and butyric acid, respectively. The conversion efficiencies for pentanoic and hexanoic acid to 1-pentanol and 1-hexanol, respectively, were 82 and 62%. C. ragsdalei also reduced acetone to 2-propanol at a conversion efficiency of 100%. Further, we showed that C. ragsdalei uses an aldehyde oxidoreductase-like enzyme to reduce n-fatty acids to the aldehyde intermediates in a reaction that requires ferredoxin and exogenous CO. Society for Industrial Microbiology and Biotechnology, 2014 Biofuels nationallicence Syngas nationallicence Acetogenesis nationallicence Aldehyde oxidoreductase nationallicence Propanol nationallicence Isom Catherine Department of Microbiology and Plant Biology, University of Oklahoma, 73019, Norman, OK, USA aut Nanny Mark School of Civil Engineering and Environmental Science, Institute for Energy and the Environment, University of Oklahoma, 73019, Norman, OK, USA aut Tanner Ralph Department of Microbiology and Plant Biology, University of Oklahoma, 73019, Norman, OK, USA aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 29-38 1367-5435 42:1<29 2015 42 10295 https://doi.org/10.1007/s10295-014-1543-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1543-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Isom Catherine Department of Microbiology and Plant Biology, University of Oklahoma, 73019, Norman, OK, USA aut NATIONALLICENCE 700 1- Nanny Mark School of Civil Engineering and Environmental Science, Institute for Energy and the Environment, University of Oklahoma, 73019, Norman, OK, USA aut NATIONALLICENCE 700 1- Tanner Ralph Department of Microbiology and Plant Biology, University of Oklahoma, 73019, Norman, OK, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 29-38 1367-5435 42:1<29 2015 42 10295