caa a22 4500 606211942 CHVBK 20210128101040.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10295-014-1545-x doi (NATIONALLICENCE)springer-10.1007/s10295-014-1545-x Immobilization of Streptomyces thermotolerans 11432 on polyurethane foam to improve production of Acetylisovaleryltylosin [Elektronische Daten] [Hongji Zhu, Weihua Wang, Jiaheng Liu, Qinggele Caiyin, Jianjun Qiao] In this study, polyurethane foam (PUF) was chemically treated to immobilize Streptomyces thermotolerans 11432 for semi-continuous production of acetylisovaleryltylosin (AIV). Based on experimental results, positive cross-linked PUF (PCPUF) was selected as the most effective carrier according to immobilized cell mass. The effect of adsorption time on immobilized mass was investigated. AIV concentration (33.54mg/l) in batch fermentations with immobilized cells was higher than with free cells (20.34mg/l). In repeated batch fermentations with immobilized S. thermotolerans 11432 using PCPUF cubes, high AIV concentrations and conversion rates were attained, ranging from 25.56 to 34.37mg/l and 79.93 to 86.31%, respectively. Significantly, this method provides a feasible strategy for efficient AIV production and offers the potential for large-scale production. Society for Industrial Microbiology and Biotechnology, 2014 Acetylisovaleryltylosin nationallicence Immobilization nationallicence Streptomyces thermotolerans nationallicence Polyurethane foam nationallicence Repeated batch fermentation nationallicence Zhu Hongji Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, China aut Wang Weihua Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, China aut Liu Jiaheng Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, China aut Caiyin Qinggele Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, China aut Qiao Jianjun Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 105-111 1367-5435 42:1<105 2015 42 10295 https://doi.org/10.1007/s10295-014-1545-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1545-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhu Hongji Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, China aut NATIONALLICENCE 700 1- Wang Weihua Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, China aut NATIONALLICENCE 700 1- Liu Jiaheng Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, China aut NATIONALLICENCE 700 1- Caiyin Qinggele Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, China aut NATIONALLICENCE 700 1- Qiao Jianjun Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, 300072, Tianjin, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 105-111 1367-5435 42:1<105 2015 42 10295