caa a22 4500 606211950 CHVBK 20210128101040.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10295-014-1542-0 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1542-0 Efficient production of (R,R)-2,3-butanediol from cellulosic hydrolysate using Paenibacillus polymyxa ICGEB2008 [Elektronische Daten] [Nidhi Adlakha, Syed Yazdani] We report here the production of pure (R,R)-2,3-butanediol (2,3-BDO) isomer by the non-pathogenic Paenibacillus polymyxa ICGEB2008 using lignocellulosic hydrolysate as substrate. Experimental design based on Plackett-Burman resulted in identification of Mn and K as most crucial salt elements along with the yeast extract for 2,3-BDO production. Further experiments using Box-Behnken design indicated that both KCl and yeast extract together had major impact on 2,3-BDO production. Optimized medium resulted in 2,3-BDO production with 2.3-fold higher maximum volumetric productivity (2.01g/L/h) and similar yield (0.33g/g sugar) as compared to rich yeast extract-peptone-dextrose medium in the bioreactor studies. Considering that the balance substrate was channeled towards ethanol, carbon recovery was close to theoretical yield between the two solvents, i.e., 2,3-BDO and ethanol. Biomass hydrolysate and corn-steep liquor was used further to produce 2,3-BDO without impacting its yield. In addition, 2,3-BDO was also produced via simultaneous saccharification and fermentation, signifying robustness of the strain. Society for Industrial Microbiology and Biotechnology, 2014 2,3-butanediol nationallicence Paenibacillus nationallicence Corn steep liquor nationallicence Biomass hydrolysate nationallicence Media optimization nationallicence Adlakha Nidhi Synthetic Biology and Biofuels Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, 10504, 110067, New Delhi, India aut Yazdani Syed Synthetic Biology and Biofuels Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, 10504, 110067, New Delhi, India aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 21-28 1367-5435 42:1<21 2015 42 10295 https://doi.org/10.1007/s10295-014-1542-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1542-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Adlakha Nidhi Synthetic Biology and Biofuels Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, 10504, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Yazdani Syed Synthetic Biology and Biofuels Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), Aruna Asaf Ali Marg, 10504, 110067, New Delhi, India aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 21-28 1367-5435 42:1<21 2015 42 10295