caa a22 4500 606211969 CHVBK 20210128101040.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10295-014-1553-x doi (NATIONALLICENCE)springer-10.1007/s10295-014-1553-x A competitive quantitative polymerase chain reaction method for characterizing the population dynamics during kimchi fermentation [Elektronische Daten] [Gee-Hyun Ahn, Jin Moon, So-Yeon Shin, Won Min, Nam Han, Jin-Ho Seo] The aim of this study was to develop a competitive quantitative-PCR (CQ-PCR) method for rapid analysis of the population dynamics of lactic acid bacteria (LAB) in kimchi. For this, whole chromosome sequences of Leuconostoc mesenteroides, Lactobacillus plantarum, and Lb. brevis were compared and species-specific PCR primers targeting dextransucrase, 16S rRNA, and surface layer protein D (SlpD) genes, respectively, were constructed. The tested strains were quantified both in medium and kimchi by CQ-PCR and the results were compared with the data obtained using a conventional plate-counting method. As a result, the three species were successfully detected and quantified by the indicated primer sets. Our results show that the CQ-PCR method targeting species-specific genes is suitable for rapid estimation of LAB population to be used in the food fermentation industry. Society for Industrial Microbiology and Biotechnology, 2014 Leuconostoc mesenteroides nationallicence Lactobacillus plantarum nationallicence Lactobacillus brevis nationallicence Kimchi nationallicence CQ-PCR nationallicence Ahn Gee-Hyun Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, 151-921, Seoul, South Korea aut Moon Jin Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, 361-763, Cheongju, South Korea aut Shin So-Yeon Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, 361-763, Cheongju, South Korea aut Min Won Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, 151-921, Seoul, South Korea aut Han Nam Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, 361-763, Cheongju, South Korea aut Seo Jin-Ho Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, 151-921, Seoul, South Korea aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 49-55 1367-5435 42:1<49 2015 42 10295 https://doi.org/10.1007/s10295-014-1553-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1553-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ahn Gee-Hyun Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, 151-921, Seoul, South Korea aut NATIONALLICENCE 700 1- Moon Jin Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, 361-763, Cheongju, South Korea aut NATIONALLICENCE 700 1- Shin So-Yeon Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, 361-763, Cheongju, South Korea aut NATIONALLICENCE 700 1- Min Won Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, 151-921, Seoul, South Korea aut NATIONALLICENCE 700 1- Han Nam Division of Animal, Horticultural, and Food Sciences, Chungbuk National University, 361-763, Cheongju, South Korea aut NATIONALLICENCE 700 1- Seo Jin-Ho Department of Agricultural Biotechnology and Center for Food and Bioconvergence, Seoul National University, 151-921, Seoul, South Korea aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 49-55 1367-5435 42:1<49 2015 42 10295