caa a22 4500 606211985 CHVBK 20210128101040.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10295-014-1534-0 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1534-0 Direct starch fermentation to l -lactic acid by a newly isolated thermophilic strain, Bacillus sp. MC-07 [Elektronische Daten] [Pramod Poudel, Yukihiro Tashiro, Hirokuni Miyamoto, Hisashi Miyamoto, Yuki Okugawa, Kenji Sakai] A newly isolated Bacillus sp. MC-07 showed 99.2% 16SrRNA gene sequence similarity with the Bacillus thermoamylovorans LMG 18084T. It demonstrated optimum and maximum growth temperatures of 50 and 62°C, respectively. The ability of MC-07 to produce optically pure l-lactic acid via direct fermentation of starch without enzymatic hydrolysis was investigated at different pH values (6.0-8.0) by intermittent adjustments every 12h. During batch fermentation in mineral salt medium containing 0.001% yeast extract at pH 7.0, 20g/L of soluble starch was utilized to produce 16.6g/L l-lactic acid at 50°C within 24h of fermentation, with 100% optical purity, 92.1% lactic acid selectivity, and an l-lactic acid yield of 0.977g/g. Direct starch fermentation at pHs 6.0, 6.5, 7.5, and 8.0 resulted in considerably lower concentrations of lactic acid than didat pH 7.0. Compared with B. thermoamylovorans LMG18084T, the ability of strain MC-07 to produce l-lactic acid was superior. Society for Industrial Microbiology and Biotechnology, 2014 Thermophilic fermentation nationallicence Bacillus sp. MC-07 nationallicence Starch nationallicence Direct lactic acid fermentation nationallicence Poudel Pramod Laboratory of Soil Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Tashiro Yukihiro Laboratory of Soil Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Miyamoto Hirokuni Japan Eco-Science (Nikkan Kagaku) Co. Ltd, 11-1-2F Shiomigaokacho, Chuo-ku, 260-0034, Chiba, Japan aut Miyamoto Hisashi Sermas Co. Ltd, Ichikawa minami 2-8-8, Ichikawa, 272-0033, Chiba, Japan aut Okugawa Yuki Laboratory of Soil Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Sakai Kenji Laboratory of Soil Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 143-149 1367-5435 42:1<143 2015 42 10295 https://doi.org/10.1007/s10295-014-1534-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1534-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Poudel Pramod Laboratory of Soil Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Tashiro Yukihiro Laboratory of Soil Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Miyamoto Hirokuni Japan Eco-Science (Nikkan Kagaku) Co. Ltd, 11-1-2F Shiomigaokacho, Chuo-ku, 260-0034, Chiba, Japan aut NATIONALLICENCE 700 1- Miyamoto Hisashi Sermas Co. Ltd, Ichikawa minami 2-8-8, Ichikawa, 272-0033, Chiba, Japan aut NATIONALLICENCE 700 1- Okugawa Yuki Laboratory of Soil Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 700 1- Sakai Kenji Laboratory of Soil Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Japan aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/1(2015-01-01), 143-149 1367-5435 42:1<143 2015 42 10295