caa a22 4500 606211993 CHVBK 20210128101040.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10295-015-1607-8 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1607-8 Efficient production and evaluation of lignocellulolytic enzymes using a constitutive protein expression system in Penicillium oxalicum [Elektronische Daten] [Yibo Hu, Haizhao Xue, Guodong Liu, Xin Song, Yinbo Qu] Native lignocellulolytic enzyme systems secreted by filamentous fungi can be further optimized by protein engineering or supplementation of exogenous enzyme components. We developed a protein production and evaluation system in cellulase-producing fungus Penicillium oxalicum. First, by deleting the major amylase gene amy15A, a strain Δ15A producing few extracellular proteins on starch was constructed. Then, three lignocellulolytic enzymes (BGL4, Xyn10B, and Cel12A) with originally low expression levels were successfully expressed with selected constitutive promoters in strain Δ15A. BGL4 and Cel12A overexpression resulted in increased specific filter paper activity (FPA), while the overexpression of Xyn10B improved volumetric FPA but not specific FPA. By switching the culture medium, this platform is convenient to produce originally low-expressed lignocellulolytic enzymes in relatively high purities on starch and to evaluate the effect of their supplementation on the performance of a complex cellulase system on cellulose. Society for Industrial Microbiology and Biotechnology, 2015 Homologous expression nationallicence Strong promoter nationallicence Cellulase nationallicence Lignocellulolytic enzyme nationallicence Penicillium oxalicum nationallicence Hu Yibo State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, 250100, Jinan, Shandong, People's Republic of China aut Xue Haizhao State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, 250100, Jinan, Shandong, People's Republic of China aut Liu Guodong State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, 250100, Jinan, Shandong, People's Republic of China aut Song Xin State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, 250100, Jinan, Shandong, People's Republic of China aut Qu Yinbo State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, 250100, Jinan, Shandong, People's Republic of China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/6(2015-06-01), 877-887 1367-5435 42:6<877 2015 42 10295 https://doi.org/10.1007/s10295-015-1607-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1607-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Yibo State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, 250100, Jinan, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Xue Haizhao State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, 250100, Jinan, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Liu Guodong State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, 250100, Jinan, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Song Xin State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, 250100, Jinan, Shandong, People's Republic of China aut NATIONALLICENCE 700 1- Qu Yinbo State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Shan Da Nan Road 27, 250100, Jinan, Shandong, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/6(2015-06-01), 877-887 1367-5435 42:6<877 2015 42 10295