caa a22 4500 606212019 CHVBK 20210128101041.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10295-015-1592-y doi (NATIONALLICENCE)springer-10.1007/s10295-015-1592-y The Methanosarcina acetivorans thioredoxin system activates DNA binding of the redox-sensitive transcriptional regulator MsvR [Elektronische Daten] [Ryan Sheehan, Addison McCarver, Catherine Isom, Elizabeth Karr, Daniel Lessner] The production of biogas (methane) by an anaerobic digestion is an important facet to renewable energy, but is subject to instability due to the sensitivity of strictly anaerobic methanogenic archaea (methanogens) to environmental perturbations, such as oxygen. An understanding of the oxidant-sensing mechanisms used by methanogens may lead to the development of more oxidant tolerant (i.e., stable) methanogen strains. MsvR is a redox-sensitive transcriptional regulator that is found exclusively in methanogens. We show here that oxidation of MsvR from Methanosarcina acetivorans (MaMsvR) with hydrogen peroxide oxidizes cysteine thiols, which inactivates MaMsvR binding to its own promoter (P msvR ). Incubation of oxidized MaMsvR with the M. acetivorans thioredoxin system (NADPH, MaTrxR, and MaTrx7) results in reduction of the cysteines back to thiols and activation of P msvR binding. These data confirm that cysteines are critical for the thiol-disulfide regulation of P msvR binding by MaMsvR and support a role for the M. acetivorans thioredoxin system in the in vivo activation of MaMsvR. The results support the feasibility of using MaMsvR and P msvR , along with the Methanosarcina genetic system, to design methanogen strains with oxidant-regulated gene expression systems, which may aid in stabilizing anaerobic digestion. Society for Industrial Microbiology and Biotechnology, 2015 Anaerobe nationallicence Methane nationallicence Methanogen nationallicence Oxidative stress nationallicence Transcription nationallicence Disulfide nationallicence Sheehan Ryan Department of Biological Sciences, University of Arkansas-Fayetteville, 72701, Fayetteville, AR, USA aut McCarver Addison Department of Biological Sciences, University of Arkansas-Fayetteville, 72701, Fayetteville, AR, USA aut Isom Catherine Department of Microbiology and Plant Biology, University of Oklahoma, 73019, Norman, OK, USA aut Karr Elizabeth Department of Microbiology and Plant Biology, University of Oklahoma, 73019, Norman, OK, USA aut Lessner Daniel Department of Biological Sciences, University of Arkansas-Fayetteville, 72701, Fayetteville, AR, USA aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/6(2015-06-01), 965-969 1367-5435 42:6<965 2015 42 10295 https://doi.org/10.1007/s10295-015-1592-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1592-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sheehan Ryan Department of Biological Sciences, University of Arkansas-Fayetteville, 72701, Fayetteville, AR, USA aut NATIONALLICENCE 700 1- McCarver Addison Department of Biological Sciences, University of Arkansas-Fayetteville, 72701, Fayetteville, AR, USA aut NATIONALLICENCE 700 1- Isom Catherine Department of Microbiology and Plant Biology, University of Oklahoma, 73019, Norman, OK, USA aut NATIONALLICENCE 700 1- Karr Elizabeth Department of Microbiology and Plant Biology, University of Oklahoma, 73019, Norman, OK, USA aut NATIONALLICENCE 700 1- Lessner Daniel Department of Biological Sciences, University of Arkansas-Fayetteville, 72701, Fayetteville, AR, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/6(2015-06-01), 965-969 1367-5435 42:6<965 2015 42 10295