caa a22 4500 606212051 CHVBK 20210128101041.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10295-015-1616-7 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1616-7 l -sorbose is not only a substrate for 2-keto- l -gulonic acid production in the artificial microbial ecosystem of two strains mixed fermentation [Elektronische Daten] [Mandlaa,, Weichao Yang, Chengbin Liu, Hui Xu] The co-culture system of the fermentation process of vitamin C can be regarded as an artificial microbial ecosystem (AME). To extend our understanding of this AME, an investigation of the relationship between strains, substrate and product was carried out in this study. The results showed that both Ketogulonicigenium vulgare and 2-keto-l-gulonic acid (2-KLG, the precursor of vitamin C) can inhibit the growth of the helper strain, while the helper strain promoted the growth of K. vulgare and 2-KLG production. Moreover, l-sorbose is not only a substrate for 2-KLG production in the AME, but also a promoter of K. vulgare and an inhibitor of the helper strain. In the earlier stage of fermentation, the inhibition of l-sorbose on the helper strain's growth is a key factor for ensuring an efficient fermentation. In the condition of adding the extra helper strain (OD: 0.57, ratio of inoculation: 2%), the yields of 2-KLG is increased by 9% in the 14% l-sorbose medium. To the best of our knowledge, this is the first report about the inhibition of substrate in the AME of 2-KLG production. Society for Industrial Microbiology and Biotechnology, 2015 Artificial microbial ecosystem nationallicence Vitamin C nationallicence 2-Keto- l -gulonic acid nationallicence l -sorbose nationallicence Helper strain nationallicence Ketogulonicigenium vulgare nationallicence Mandlaa, Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, 110016, Shenyang, China aut Yang Weichao Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, 110016, Shenyang, China aut Liu Chengbin Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, 110016, Shenyang, China aut Xu Hui Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, 110016, Shenyang, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/6(2015-06-01), 897-904 1367-5435 42:6<897 2015 42 10295 https://doi.org/10.1007/s10295-015-1616-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1616-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mandlaa Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Yang Weichao Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Liu Chengbin Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Xu Hui Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/6(2015-06-01), 897-904 1367-5435 42:6<897 2015 42 10295