caa a22 4500 606212094 CHVBK 20210128101041.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10295-015-1612-y doi (NATIONALLICENCE)springer-10.1007/s10295-015-1612-y Whole-genome sequencing of Bacillus subtilis XF-1 reveals mechanisms for biological control and multiple beneficial properties in plants [Elektronische Daten] [Shengye Guo, Xingyu Li, Pengfei He, Honhing Ho, Yixin Wu, Yueqiu He] Bacillus subtilis XF-1 is a gram-positive, plant-associated bacterium that stimulates plant growth and produces secondary metabolites that suppress soil-borne plant pathogens. In particular, it is especially highly efficient at controlling the clubroot disease of cruciferous crops. Its 4,061,186-bp genome contains an estimated 3853 protein-coding sequences and the 1155 genes of XF-1 are present in most genome-sequenced Bacillus strains: 3757 genes in B. subtilis 168, and 1164 in B. amyloliquefaciens FZB42. Analysis using the Cluster of Orthologous Groups database of proteins shows that 60 genes control bacterial mobility, 221 genes are related to cell wall and membrane biosynthesis, and more than 112 are genes associated with secondary metabolites. In addition, the genes contributed to the strain's plant colonization, bio-control and stimulation of plant growth. Sequencing of the genome is a fundamental step for developing a desired strain to serve as an efficient biological control agent and plant growth stimulator. Similar to other members of the taxon, XF-1 has a genome that contains giant gene clusters for the non-ribosomal synthesis of antifungal lipopeptides (surfactin and fengycin), the polyketides (macrolactin and bacillaene), the siderophore bacillibactin, and the dipeptide bacilysin. There are two synthesis pathways for volatile growth-promoting compounds. The expression of biosynthesized antibiotic peptides in XF-1 was revealed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Society for Industrial Microbiology and Biotechnology, 2015 Genome sequence nationallicence Bacillus subtilis XF-1 nationallicence Bio-control nationallicence PGPR nationallicence NRPs nationallicence Guo Shengye Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, 650201, Kunming, China aut Li Xingyu Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, 650201, Kunming, China aut He Pengfei Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, 650201, Kunming, China aut Ho Honhing Department of Biology, State University of New York, 12561, New Paltz, NY, USA aut Wu Yixin Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, 650201, Kunming, China aut He Yueqiu Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, 650201, Kunming, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/6(2015-06-01), 925-937 1367-5435 42:6<925 2015 42 10295 https://doi.org/10.1007/s10295-015-1612-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1612-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guo Shengye Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, 650201, Kunming, China aut NATIONALLICENCE 700 1- Li Xingyu Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, 650201, Kunming, China aut NATIONALLICENCE 700 1- He Pengfei Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, 650201, Kunming, China aut NATIONALLICENCE 700 1- Ho Honhing Department of Biology, State University of New York, 12561, New Paltz, NY, USA aut NATIONALLICENCE 700 1- Wu Yixin Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, 650201, Kunming, China aut NATIONALLICENCE 700 1- He Yueqiu Faculty of Agronomy and Biotechnology, Yunnan Agricultural University, 650201, Kunming, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/6(2015-06-01), 925-937 1367-5435 42:6<925 2015 42 10295