caa a22 4500 606212140 CHVBK 20210128101041.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10295-015-1646-1 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1646-1 Influence of glucose fermentation on CO2 assimilation to acetate in homoacetogen Blautia coccoides GA-1 [Elektronische Daten] [Chong Liu, Jianzheng Li, Yupeng Zhang, Antwi Philip, En Shi, Xue Chi, Jia Meng] Fermentation of glucose influences CO2 assimilation to acetate in homoacetogens. Blautia coccoides was investigated for a better understanding of the metabolic characteristics of homoacetogens in mixotrophic cultures. Batch cultures of the strain with H2/CO2 as a sole carbon source reached an acetate yield of 5.32g/g dry cell weight after 240h of incubation. Autotrophic metabolism was inhibited as glucose was added into the culture: the higher the glucose concentration the lower the autotrophic ability of the bacterium. Autotrophy was inhibited by high glucose concentration, probably due to the competition for coenzyme A between the Embden-Meyerhof-Parnas pathway and the Wood-Ljungdahl carbon fixation pathway, the energy (adenosine triphosphate) allocation for synthesis of cell carbon and reduction of CO2, in combination with the low pH caused by the accumulation of acetate. Society for Industrial Microbiology and Biotechnology, 2015 Homoacetogen nationallicence Mixotrophic culture nationallicence Autotrophic metabolism nationallicence Heterotrophic metabolism nationallicence Metabolic repression nationallicence Liu Chong School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China aut Li Jianzheng School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China aut Zhang Yupeng School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China aut Philip Antwi School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China aut Shi En School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China aut Chi Xue School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China aut Meng Jia School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/9(2015-09-01), 1217-1224 1367-5435 42:9<1217 2015 42 10295 https://doi.org/10.1007/s10295-015-1646-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1646-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Chong School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China aut NATIONALLICENCE 700 1- Li Jianzheng School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China aut NATIONALLICENCE 700 1- Zhang Yupeng School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China aut NATIONALLICENCE 700 1- Philip Antwi School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China aut NATIONALLICENCE 700 1- Shi En School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China aut NATIONALLICENCE 700 1- Chi Xue School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China aut NATIONALLICENCE 700 1- Meng Jia School of Municipal and Environmental Engineering, Harbin Institute of Technology, 150090, Harbin, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/9(2015-09-01), 1217-1224 1367-5435 42:9<1217 2015 42 10295