caa a22 4500 606212159 CHVBK 20210128101041.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10295-015-1628-3 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1628-3 Enhancing xylanase production in the thermophilic fungus Myceliophthora thermophila by homologous overexpression of Mtxyr1 [Elektronische Daten] [Juan Wang, Yaning Wu, Yanfen Gong, Shaowen Yu, Gang Liu] The xylanase regulator 1 protein in Myceliophthora thermophila ATCC42464 (MtXyr1) is 60% homologous with that of Trichoderma reesei. However, MtXyr1's regulatory role on cellulolytic and xylanolytic genes in M. thermophila is unknown. Herein, MtXyr1 was overexpressed under the control of the MtPpdc (pyruvate decarboxylase) promoter. Compared with the wild type, the extracellular xylanase activities of the transformant cultured in non-inducing and inducing media for 120h were 25.19- and 9.04-fold higher, respectively. The Mtxyr1 mRNA level was 300-fold higher than in the wild type in corncob-containing medium. However, the filter paper activity and endoglucanase activities were unchanged in corncob-containing medium and glucose-containing medium. The different zymograms between the transformant and the wild type were analyzed and identified by mass spectrometry as three xylanases of the glycoside hydrolase (GH) family 11. Thus, overexpression of xyr1 resulted in enhanced xylanase activity in M. thermophila. Xylanase production could be improved by overexpressing Mtxyr1 in M. thermophila. Society for Industrial Microbiology and Biotechnology, 2015 Thermophilic fungi nationallicence Xylanase nationallicence Myceliophthora thermophila nationallicence xyr1 nationallicence Homologous expression nationallicence Wang Juan Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, 518060, Shenzhen, China aut Wu Yaning Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, 518060, Shenzhen, China aut Gong Yanfen Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, 518060, Shenzhen, China aut Yu Shaowen Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, 518060, Shenzhen, China aut Liu Gang Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, 518060, Shenzhen, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/9(2015-09-01), 1233-1241 1367-5435 42:9<1233 2015 42 10295 https://doi.org/10.1007/s10295-015-1628-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1628-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Juan Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, 518060, Shenzhen, China aut NATIONALLICENCE 700 1- Wu Yaning Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, 518060, Shenzhen, China aut NATIONALLICENCE 700 1- Gong Yanfen Shenzhen Key Laboratory of Marine Bioresources and Ecology, Shenzhen University, 518060, Shenzhen, China aut NATIONALLICENCE 700 1- Yu Shaowen Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, 518060, Shenzhen, China aut NATIONALLICENCE 700 1- Liu Gang Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, 518060, Shenzhen, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/9(2015-09-01), 1233-1241 1367-5435 42:9<1233 2015 42 10295