caa a22 4500 606212167 CHVBK 20210128101041.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10295-015-1657-y doi (NATIONALLICENCE)springer-10.1007/s10295-015-1657-y Biosynthetic enhancement of single-stage Poly(3-hydroxybutyrate- co -4-hydroxybutyrate) production by manipulating the substrate mixtures [Elektronische Daten] [Kai-Hee Huong, Shantini Kannusamy, Sumithda Lim, A. Amirul] Two-stage fermentation was normally employed to achieve a high poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] productivity with higher 4HB molar fraction. Here, we demonstrated single-stage fermentation method which is more industrial feasible by implementing mixed-substrate cultivation strategy. Studies on bioreactor scale show a remarkably high PHA accumulation of 73 wt%, contributing to a high PHA concentration and product yield of 8.6g/L and 2.7g/g, respectively. This fermentation strategy has resulted in copolymers with wider range of 4HB monomer composition, which ranges from 12 to 55mol%. These copolymers show a broad range of weight average molecular weight (M w ) from 119.5 to 407.0kDa. The copolymer characteristics were found to be predominantly affected by the nature of the substrates and the mixture strategies, regardless of the 4HB monomer compositions. This was supported by the determination of copolymer randomness using 13C-NMR analysis. The study warrants significantly in the copolymer scale-up and modeling at industrial level. Society for Industrial Microbiology and Biotechnology, 2015 Copolymer P(3HB- co -4HB) nationallicence Molecular weight nationallicence Polymer randomness nationallicence Substrate mixtures nationallicence Single-stage cultivation nationallicence Huong Kai-Hee Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM, 11700, Gelugor, Penang, Malaysia aut Kannusamy Shantini School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia aut Lim Sumithda School of Chemical Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia aut Amirul A. Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM, 11700, Gelugor, Penang, Malaysia aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/9(2015-09-01), 1291-1297 1367-5435 42:9<1291 2015 42 10295 https://doi.org/10.1007/s10295-015-1657-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1657-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huong Kai-Hee Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM, 11700, Gelugor, Penang, Malaysia aut NATIONALLICENCE 700 1- Kannusamy Shantini School of Biological Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia aut NATIONALLICENCE 700 1- Lim Sumithda School of Chemical Sciences, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia aut NATIONALLICENCE 700 1- Amirul A. Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM, 11700, Gelugor, Penang, Malaysia aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/9(2015-09-01), 1291-1297 1367-5435 42:9<1291 2015 42 10295