caa a22 4500 606212213 CHVBK 20210128101041.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10295-015-1652-3 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1652-3 Batch production of coenzyme Q10 by recombinant Escherichia coli containing the decaprenyl diphosphate synthase gene from Sphingomonas baekryungensis [Elektronische Daten] [Irene Martínez, Claudia Méndez, Julio Berríos, Claudia Altamirano, Alvaro Díaz-Barrera] Coenzyme Q10 (CoQ10) is an important antioxidant used in medicine, dietary supplements, and cosmetic applications. In the present work, the production of CoQ10 using a recombinant Escherichia coli strain containing the decaprenyl diphosphate synthase from Sphingomonas baekryungensis was investigated, wherein the effects of culture medium, temperature, and agitation rate on the production process were assessed. It was found that Luria-Bertani (LB) medium was superior to M9 with glucose medium. Higher temperature (37°C) and higher agitation rate (900rpm) improved the specific CoQ10 content significantly in LB medium; on the contrary, the use of M9 medium with glucose showed similar values. Specifically, in LB medium, an increase from 300 to 900rpm in the agitation rate resulted in increases of 55 and 197% in the specific CoQ10 content and COQ10 productivity, respectively. Therefore, the results obtained in the present work are a valuable contribution for the optimization of CoQ10 production processes using recombinant E. coli strains. Society for Industrial Microbiology and Biotechnology, 2015 Coenzyme Q nationallicence Oxygen supply nationallicence Agitation rate nationallicence Recombinant Escherichia coli nationallicence Culture medium nationallicence Martínez Irene Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile aut Méndez Claudia Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile aut Berríos Julio Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile aut Altamirano Claudia Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile aut Díaz-Barrera Alvaro Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/9(2015-09-01), 1283-1289 1367-5435 42:9<1283 2015 42 10295 https://doi.org/10.1007/s10295-015-1652-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1652-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martínez Irene Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile aut NATIONALLICENCE 700 1- Méndez Claudia Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile aut NATIONALLICENCE 700 1- Berríos Julio Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile aut NATIONALLICENCE 700 1- Altamirano Claudia Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile aut NATIONALLICENCE 700 1- Díaz-Barrera Alvaro Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2085, Valparaíso, Chile aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/9(2015-09-01), 1283-1289 1367-5435 42:9<1283 2015 42 10295