caa a22 4500 606212221 CHVBK 20210128101041.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10295-015-1650-5 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1650-5 Scalable temperature induced stress for the large-scale production of functionalized Bifidobacteria [Elektronische Daten] [Huu Nguyen, Hary Razafindralambo, Aurore Richel, Nicolas Jacquet, Pol Evrard, Patrice Antoine, Philippe Thonart, Frank Delvigne] The application of sub-lethal stresses is known to be an efficient strategy to enhance survival of probiotic bacteria during drying processes. In this context, we previously showed that the application of heat stress upon the entry into stationary phase increased significantly the viability of Bifidobacterium bifidum. However, this heat shock has been considered only in small-scale bioreactor and no information is available for a possible scaling-up strategy. Five different operating scales (0.2, 2, 20, 200 and 2000 L) have thus been tested and the results showed that the viability of B. bifidum increases from 3.15 to 6.57 folds, depending on the scale considered. Our observations pointed out the fact that the heat stress procedure is scalable according to the main outcome, i.e., increases in cell viability, but other factors have to be taken into account. Among these factors, dissolved carbon dioxide seems to play a significant role, since it explains the differences observed between the test performed at laboratory scale and in industrial conditions. Society for Industrial Microbiology and Biotechnology, 2015 Exopolysaccharide nationallicence Scale-up nationallicence Bioreactor nationallicence Heat stress nationallicence Probiotics nationallicence Carbon dioxide nationallicence Bifidobacteria nationallicence Cfu : Colony-forming unit nationallicence EPS : Exopolysaccharide nationallicence GC : Gas chromatography nationallicence LAB : Lactic acid bacteria nationallicence MRS : Man-Rogosa-Sharpe medium nationallicence RCM : Reinforced clostridial medium nationallicence L : Litre nationallicence HPLC : High-performance liquid chromatography nationallicence PBS : Phosphate buffer saline nationallicence Nguyen Huu Groupe de Biochimie industrielle and Produits naturels, Université de Ton Duc Thang, 19 Nguyen Huu Tho, Tan Phong, Dis. 7, Ho Chi Minh Ville, Vietnam aut Razafindralambo Hary Unité de Science des aliments et formulation, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030, Gembloux, Belgium aut Richel Aurore Compagnie de THT s.a, Parc Scientifique CREALYS, Camille Hubert 17, 5032, Gembloux, Belgium aut Jacquet Nicolas Compagnie de THT s.a, Parc Scientifique CREALYS, Camille Hubert 17, 5032, Gembloux, Belgium aut Evrard Pol Unité de chimie biologique industrielle, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030, Gembloux, Belgium aut Antoine Patrice Unité de chimie biologique industrielle, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030, Gembloux, Belgium aut Thonart Philippe Microbial Processes and Interactions (MiPI), Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030, Gembloux, Belgium aut Delvigne Frank Microbial Processes and Interactions (MiPI), Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030, Gembloux, Belgium aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/9(2015-09-01), 1225-1231 1367-5435 42:9<1225 2015 42 10295 https://doi.org/10.1007/s10295-015-1650-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1650-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nguyen Huu Groupe de Biochimie industrielle and Produits naturels, Université de Ton Duc Thang, 19 Nguyen Huu Tho, Tan Phong, Dis. 7, Ho Chi Minh Ville, Vietnam aut NATIONALLICENCE 700 1- Razafindralambo Hary Unité de Science des aliments et formulation, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Richel Aurore Compagnie de THT s.a, Parc Scientifique CREALYS, Camille Hubert 17, 5032, Gembloux, Belgium aut NATIONALLICENCE 700 1- Jacquet Nicolas Compagnie de THT s.a, Parc Scientifique CREALYS, Camille Hubert 17, 5032, Gembloux, Belgium aut NATIONALLICENCE 700 1- Evrard Pol Unité de chimie biologique industrielle, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Antoine Patrice Unité de chimie biologique industrielle, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Thonart Philippe Microbial Processes and Interactions (MiPI), Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030, Gembloux, Belgium aut NATIONALLICENCE 700 1- Delvigne Frank Microbial Processes and Interactions (MiPI), Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030, Gembloux, Belgium aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/9(2015-09-01), 1225-1231 1367-5435 42:9<1225 2015 42 10295