caa a22 4500 60621223X CHVBK 20210128101041.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10295-015-1649-y doi (NATIONALLICENCE)springer-10.1007/s10295-015-1649-y Exploring medium-chain-length polyhydroxyalkanoates production in the engineered yeast Yarrowia lipolytica [Elektronische Daten] [Cuijuan Gao, Qingsheng Qi, Catherine Madzak, Carol Lin] Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) are a large class of biopolymers that have attracted extensive attention as renewable and biodegradable bio-plastics. They are naturally synthesized via fatty acid de novo biosynthesis pathway or β-oxidation pathway from Pseudomonads. The unconventional yeast Yarrowia lipolytica has excellent lipid/fatty acid catabolism and anabolism capacity depending of the mode of culture. Nevertheless, it cannot naturally synthesize PHA, as it does not express an intrinsic PHA synthase. Here, we constructed a genetically modified strain of Y. lipolytica by heterologously expressing PhaC1gene from P.aeruginosa PAO1 with a PTS1 peroxisomal signal. When in single copy, the codon optimized PhaC1 allowed the synthesis of 0.205% DCW of PHA after 72h cultivation in YNBD medium containing 0.1% oleic acid. By using a multi-copy integration strategy, PHA content increased to 2.84% DCW when the concentration of oleic acid in YNBD was 1.0%. Furthermore, when the recombinant yeast was grown in the medium containing triolein, PHA accumulated up to 5.0% DCW with as high as 21.9g/L DCW, which represented 1.11g/L in the culture. Our results demonstrated the potential use of Y. lipolytica as a promising microbial cell factory for PHA production using food waste, which contains lipids and other essential nutrients. Society for Industrial Microbiology and Biotechnology, 2015 Medium-chain-length polyhydroxyalkanoates nationallicence Yarrowia lipolytica nationallicence PHA synthase nationallicence Strain engineering nationallicence Triolein nationallicence Gao Cuijuan School of Life Science, Linyi University, 276005, Linyi, People's Republic of China aut Qi Qingsheng State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut Madzak Catherine INRA, UMR1319, Micalis Institute, AgroParisTech Campus, CBAI, 78850, Thiverval-Grignon, France aut Lin Carol School of Energy and Environment, City University of Hong Kong, Kowloon, Hong Kong aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/9(2015-09-01), 1255-1262 1367-5435 42:9<1255 2015 42 10295 https://doi.org/10.1007/s10295-015-1649-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1649-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gao Cuijuan School of Life Science, Linyi University, 276005, Linyi, People's Republic of China aut NATIONALLICENCE 700 1- Qi Qingsheng State Key Laboratory of Microbial Technology, Shandong University, 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Madzak Catherine INRA, UMR1319, Micalis Institute, AgroParisTech Campus, CBAI, 78850, Thiverval-Grignon, France aut NATIONALLICENCE 700 1- Lin Carol School of Energy and Environment, City University of Hong Kong, Kowloon, Hong Kong aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/9(2015-09-01), 1255-1262 1367-5435 42:9<1255 2015 42 10295