caa a22 4500 606212248 CHVBK 20210128101042.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10295-015-1624-7 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1624-7 Enhanced production of l -sorbose in an industrial Gluconobacter oxydans strain by identification of a strong promoter based on proteomics analysis [Elektronische Daten] [Yudong Hu, Hui Wan, Jianghua Li, Jingwen Zhou] Gluconobacter oxydans is capable of rapidly incomplete oxidation of many sugars and alcohols, which means the strain has great potential for industrial purposes. Strong promoters are one of the essential factors that can improve strain performance by overexpression of specific genes. In this study, a pipeline for screening strong promoters by proteomics analysis was established. Based on the procedure, a new strong promoter designated as P B932_2000 was identified in G. oxydans WSH-003. The promoter region was characterized based on known genome sequence information using BPROM. The strength of P B932_2000 was further assessed by analysis of enhanced green fluorescent protein (egfp) expression and comparison with egfp expression by two commonly used strong promoters, P E. coli_tufB and P G. oxydans_tufB . Both quantitative real-time PCR and fluorescence intensities for egfp gene expression showed that P B932_2000 promoter is stronger than the other two. Overexpression of d-sorbitol dehydrogenase (sldh) by P B932_2000 in G. oxydans WSH-003 enhanced the titer and productivity of l-sorbose synthesis from d-sorbitol by 12.0% and 33.3%, respectively. These results showed that proteomics analysis is an efficient way to identify strong promoters. The isolated promoter P B932_2000 could further facilitate the metabolic engineering of G. oxydans. Society for Industrial Microbiology and Biotechnology, 2015 l -ascorbic acid nationallicence Acetic acid bacteria nationallicence Constitutive promoter nationallicence Metabolic engineering nationallicence d -sorbitol nationallicence Hu Yudong Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut Wan Hui Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut Li Jianghua Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut Zhou Jingwen Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/7(2015-07-01), 1039-1047 1367-5435 42:7<1039 2015 42 10295 https://doi.org/10.1007/s10295-015-1624-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1624-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Yudong Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Wan Hui Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Li Jianghua Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Zhou Jingwen Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, Jiangsu, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/7(2015-07-01), 1039-1047 1367-5435 42:7<1039 2015 42 10295