caa a22 4500 606212280 CHVBK 20210128101042.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10295-015-1622-9 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1622-9 Engineering microaerobic metabolism of E. coli for 1,2-propanediol production [Elektronische Daten] [Rachit Jain, Jin Huang, Qipeng Yuan, Yajun Yan] Establishment of novel metabolic pathways for biosynthesis of chemicals, fuels and pharmaceuticals has been demonstrated in Escherichia coli due to its ease of genetic manipulation and adaptability to varying oxygen levels. E. coli growing under microaerobic condition is known to exhibit features of both aerobic and anaerobic metabolism. In this work, we attempt to engineer this metabolism for production of 1,2-propanediol. We first redirect the carbon flux by disrupting carbon-competing pathways to increase the production of 1,2-propanediol microaerobically from 0.25 to 0.85g/L. We then disrupt the first committed step of E. coli's ubiquinone biosynthesis pathway (ubiC) to prevent the oxidation of NADH in microaerobic conditions. Coupling this strategy with carbon flux redirection leads to enhanced production of 1,2-propanediol at 1.2g/L. This work demonstrates the production of non-native reduced chemicals in E. coli by engineering its microaerobic metabolism. Society for Industrial Microbiology and Biotechnology, 2015 1,2-Propanediol nationallicence Microaerobic nationallicence Ubiquinone nationallicence Escherichia coli nationallicence Jain Rachit College of Engineering, University of Georgia, 30602, Athens, GA, USA aut Huang Jin State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 100029, Beijing, China aut Yuan Qipeng State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 100029, Beijing, China aut Yan Yajun BioChemical Engineering Program, 615 Driftmier Engineering Center, College of Engineering, University of Georgia, 30602, Athens, GA, USA aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/7(2015-07-01), 1049-1055 1367-5435 42:7<1049 2015 42 10295 https://doi.org/10.1007/s10295-015-1622-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1622-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jain Rachit College of Engineering, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 700 1- Huang Jin State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 100029, Beijing, China aut NATIONALLICENCE 700 1- Yuan Qipeng State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 100029, Beijing, China aut NATIONALLICENCE 700 1- Yan Yajun BioChemical Engineering Program, 615 Driftmier Engineering Center, College of Engineering, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/7(2015-07-01), 1049-1055 1367-5435 42:7<1049 2015 42 10295