caa a22 4500 606212299 CHVBK 20210128101042.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10295-015-1630-9 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1630-9 Increasing succinic acid production using the PTS-independent glucose transport system in a Corynebacterium glutamicum PTS-defective mutant [Elektronische Daten] [Zhihui Zhou, Chen Wang, Hongtao Xu, Zhongjun Chen, Heng Cai] Succinic acid synthesized from glucose shows potential as a bio-based platform chemical. However, the need for a high glucose concentration, and the accompanying low yields, limit its industrial applications. Despite efficient glucose uptake by the phosphotransferase system (PTS), 1mol of phosphoenolpyruvate is required for each mole of internalized glucose. Therefore, a PTS-defective Corynebacterium glutamicum mutant was constructed to increase phosphoenolpyruvate availability for succinic acid synthesis, resulting in a lower glucose utilization rate and slower growth. The transcriptional regulator iolR was also deleted to enable the PTS-defective mutant to utilize glucose via iolT-mediated glucose transport. Deletion of iolR and overexpression of iolT1 and ppgk (polyphosphate glucokinase) in the PTS-deficient C. glutamicum strain completely restored glucose utilization, increasing production by 11.6% and yield by 32.4% compared with the control. This study revealed for the first time that iolR represses the expression of the two glucokinase genes (glk and ppgk). Society for Industrial Microbiology and Biotechnology, 2015 Phosphoenolpyruvic acid nationallicence Corynebacterium glutamicum nationallicence PTS-independent glucose uptake genes nationallicence Transcriptional regulator nationallicence Succinic acid nationallicence Zhou Zhihui College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 211816, Nanjing, China aut Wang Chen College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 211816, Nanjing, China aut Xu Hongtao College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 211816, Nanjing, China aut Chen Zhongjun College of Food Science and Engineering, Inner Mongolia Agricultural University, 010018, Hohhot, China aut Cai Heng College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 211816, Nanjing, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/7(2015-07-01), 1073-1082 1367-5435 42:7<1073 2015 42 10295 https://doi.org/10.1007/s10295-015-1630-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1630-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Zhihui College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 211816, Nanjing, China aut NATIONALLICENCE 700 1- Wang Chen College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 211816, Nanjing, China aut NATIONALLICENCE 700 1- Xu Hongtao College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 211816, Nanjing, China aut NATIONALLICENCE 700 1- Chen Zhongjun College of Food Science and Engineering, Inner Mongolia Agricultural University, 010018, Hohhot, China aut NATIONALLICENCE 700 1- Cai Heng College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 211816, Nanjing, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/7(2015-07-01), 1073-1082 1367-5435 42:7<1073 2015 42 10295