caa a22 4500 606212345 CHVBK 20210128101042.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10295-015-1698-2 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1698-2 Studies on properties of the xylan-binding domain and linker sequence of xylanase XynG1-1 from Paenibacillus campinasensis G1-1 [Elektronische Daten] [Yihan Liu, Lin Huang, Weiguo Li, Wei Guo, Hongchen Zheng, Jianling Wang, Fuping Lu] Xylanase XynG1-1 from Paenibacillus campinasensis G1-1 consists of a catalytic domain (CD), a family 6_36 carbohydrate-binding module which is a xylan-binding domain (XBD), and a linker sequence (LS) between them. The structure of XynG1-3 from Bacillus pumilus G1-3 consists only of a CD. To investigate the functions and properties of the XBD and LS of XynG1-1, two truncated forms (XynG1-1CDL, XynG1-1CD) and three fusion derivatives (XynG1-3CDL, XynG1-3CDX and XynG1-3CDLX) were constructed and biochemically characterized. The optimum conditions for the catalytic activity of mutants of XynG1-1 and XynG1-3 were 60°C and pH7.0, and 55°C and pH8.0, respectively, the same as for the corresponding wild-type enzymes. XynGs with an XBD were stable over a broad temperature (30-80°C) and pH range (4.0-11.0), respectively, on incubation for 3h. Kinetic parameters (K m , k cat, k cat/K m ) of XynGs were determined with soluble birchwood xylan and insoluble oat spelt xylan as substrates. XynGs with the XBD showed better affinities toward, and more efficient catalysis of hydrolysis of the insoluble substrate. The XBD had positive effects on thermostability and pH stability and a crucial function in the ability of the enzyme to bind and hydrolyze insoluble substrate. The LS had little effect on the overall stability of the xylanase and no relationship with affinities for soluble and insoluble substrates or catalytic efficiency. Society for Industrial Microbiology and Biotechnology, 2015 Xylanase nationallicence Carbohydrate-binding module nationallicence Xylan-binding domain nationallicence Linker sequence nationallicence Enzyme properties nationallicence Liu Yihan Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, 300457, Tianjin, China aut Huang Lin Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, 300457, Tianjin, China aut Li Weiguo Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, 300457, Tianjin, China aut Guo Wei Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, 300457, Tianjin, China aut Zheng Hongchen Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, 300457, Tianjin, China aut Wang Jianling Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, 300457, Tianjin, China aut Lu Fuping Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, 300457, Tianjin, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/12(2015-12-01), 1591-1599 1367-5435 42:12<1591 2015 42 10295 https://doi.org/10.1007/s10295-015-1698-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1698-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Yihan Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, 300457, Tianjin, China aut NATIONALLICENCE 700 1- Huang Lin Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, 300457, Tianjin, China aut NATIONALLICENCE 700 1- Li Weiguo Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, 300457, Tianjin, China aut NATIONALLICENCE 700 1- Guo Wei Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, 300457, Tianjin, China aut NATIONALLICENCE 700 1- Zheng Hongchen Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, 300457, Tianjin, China aut NATIONALLICENCE 700 1- Wang Jianling Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, 300457, Tianjin, China aut NATIONALLICENCE 700 1- Lu Fuping Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, 300457, Tianjin, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/12(2015-12-01), 1591-1599 1367-5435 42:12<1591 2015 42 10295