caa a22 4500 606212353 CHVBK 20210128101042.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10295-015-1697-3 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1697-3 Application of enzymatic apple pomace hydrolysate to production of 2,3-butanediol by alkaliphilic Bacillus licheniformis NCIMB 8059 [Elektronische Daten] [Aneta Białkowska, Ewa Gromek, Joanna Krysiak, Barbara Sikora, Halina Kalinowska, Marzena Jędrzejczak-Krzepkowska, Celina Kubik, Siegmund Lang, Fokko Schütt, Marianna Turkiewicz] 2,3-Butanediol (2,3-BD) synthesis by a nonpathogenic bacterium Bacillus licheniformis NCIMB 8059 from enzymatic hydrolysate of depectinized apple pomace and its blend with glucose was studied. In shake flasks, the maximum diol concentration in fed-batch fermentations was 113g/L (in 163h, from the hydrolysate, feedings with glucose) while in batch processes it was around 27g/L (in 32h, from the hydrolysate and glucose blend). Fed-batch fermentations in the 0.75 and 30 L fermenters yielded 87.71g/L 2,3-BD in 160h, and 72.39g/L 2,3-BD in 94h, respectively (from the hydrolysate and glucose blend, feedings with glucose). The hydrolysate of apple pomace, which was for the first time used for microbial 2,3-BD production is not only a source of sugars but also essential minerals. Society for Industrial Microbiology and Biotechnology, 2015 2,3-Butanediol nationallicence Apple pomace hydrolysates nationallicence Bacillus licheniformis nationallicence Fermentation nationallicence Białkowska Aneta Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut Gromek Ewa Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut Krysiak Joanna Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut Sikora Barbara Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut Kalinowska Halina Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut Jędrzejczak-Krzepkowska Marzena Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut Kubik Celina Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut Lang Siegmund Institute of Biochemistry and Biotechnology, Technical University Braunschweig, Spielmannstrasse 7, 38106, Brunswick, Germany aut Schütt Fokko Thünen Institute of Wood Research, Leuschnerstrasse 91, 21031, Hamburg, Germany aut Turkiewicz Marianna Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/12(2015-12-01), 1609-1621 1367-5435 42:12<1609 2015 42 10295 https://doi.org/10.1007/s10295-015-1697-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1697-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Białkowska Aneta Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut NATIONALLICENCE 700 1- Gromek Ewa Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut NATIONALLICENCE 700 1- Krysiak Joanna Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut NATIONALLICENCE 700 1- Sikora Barbara Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut NATIONALLICENCE 700 1- Kalinowska Halina Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut NATIONALLICENCE 700 1- Jędrzejczak-Krzepkowska Marzena Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut NATIONALLICENCE 700 1- Kubik Celina Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut NATIONALLICENCE 700 1- Lang Siegmund Institute of Biochemistry and Biotechnology, Technical University Braunschweig, Spielmannstrasse 7, 38106, Brunswick, Germany aut NATIONALLICENCE 700 1- Schütt Fokko Thünen Institute of Wood Research, Leuschnerstrasse 91, 21031, Hamburg, Germany aut NATIONALLICENCE 700 1- Turkiewicz Marianna Institute of Technical Biochemistry, Lodz University of Technology, Stefanowskiego Street 4/10, 90-924, Lodz, Poland aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/12(2015-12-01), 1609-1621 1367-5435 42:12<1609 2015 42 10295