caa a22 4500 60621237X CHVBK 20210128101042.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10295-015-1695-5 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1695-5 Increased ethanol production by deletion of HAP4 in recombinant xylose-assimilating Saccharomyces cerevisiae [Elektronische Daten] [Akinori Matsushika, Tamotsu Hoshino] The Saccharomyces cerevisiae HAP4gene encodes a transcription activator that plays a key role in controlling the expression of genes involved in mitochondrial respiration and reductive pathways. This work examines the effect of knockout of the HAP4 gene on aerobic ethanol production in a xylose-utilizing S. cerevisiae strain. A hap4-deleted recombinant yeast strain (B42-DHAP4) showed increased maximum concentration, production rate, and yield of ethanol compared with the reference strain MA-B42, irrespective of cultivation medium (glucose, xylose, or glucose/xylose mixtures). Notably, B42-DHAP4 was capable of producing ethanol from xylose as the sole carbon source under aerobic conditions, whereas no ethanol was produced by MA-B42. Moreover, the rate of ethanol production and ethanol yield (0.44 g/g) from the detoxified hydrolysate of wood chips was markedly improved in B42-DHAP4 compared to MA-B42. Thus, the results of this study support the view that deleting HAP4 in xylose-utilizing S. cerevisiae strains represents a useful strategy in ethanol production processes. Society for Industrial Microbiology and Biotechnology, 2015 Saccharomyces cerevisiae nationallicence HAP4 gene nationallicence Xylose nationallicence Ethanol production nationallicence Lignocellulosic hydrolysate nationallicence Matsushika Akinori Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, 739-0046, Hiroshima, Japan aut Hoshino Tamotsu Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, 739-0046, Hiroshima, Japan aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/12(2015-12-01), 1623-1631 1367-5435 42:12<1623 2015 42 10295 https://doi.org/10.1007/s10295-015-1695-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1695-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsushika Akinori Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, 739-0046, Hiroshima, Japan aut NATIONALLICENCE 700 1- Hoshino Tamotsu Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, 739-0046, Hiroshima, Japan aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/12(2015-12-01), 1623-1631 1367-5435 42:12<1623 2015 42 10295