caa a22 4500 606212418 CHVBK 20210128101042.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10295-014-1541-1 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1541-1 An integrated biotechnology platform for developing sustainable chemical processes [Elektronische Daten] [Nelson Barton, Anthony Burgard, Mark Burk, Jason Crater, Robin Osterhout, Priti Pharkya, Brian Steer, Jun Sun, John Trawick, Stephen Van Dien, Tae Yang, Harry Yim] Abstract : Genomatica has established an integrated computational/experimental metabolic engineering platform to design, create, and optimize novel high performance organisms and bioprocesses. Here we present our platform and its use to develop E. coli strains for production of the industrial chemical 1,4-butanediol (BDO) from sugars. A series of examples are given to demonstrate how a rational approach to strain engineering, including carefully designed diagnostic experiments, provided critical insights about pathway bottlenecks, byproducts, expression balancing, and commercial robustness, leading to a superior BDO production strain and process. Society for Industrial Microbiology and Biotechnology, 2014 1,4-butanediol nationallicence Metabolic engineering nationallicence Modeling nationallicence Fermentation nationallicence Barton Nelson Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut Burgard Anthony Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut Burk Mark Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut Crater Jason Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut Osterhout Robin Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut Pharkya Priti Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut Steer Brian Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut Sun Jun Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut Trawick John Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut Van Dien Stephen Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut Yang Tae Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut Yim Harry Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/3(2015-03-01), 349-360 1367-5435 42:3<349 2015 42 10295 https://doi.org/10.1007/s10295-014-1541-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1541-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barton Nelson Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Burgard Anthony Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Burk Mark Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Crater Jason Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Osterhout Robin Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Pharkya Priti Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Steer Brian Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Sun Jun Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Trawick John Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Van Dien Stephen Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Yang Tae Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut NATIONALLICENCE 700 1- Yim Harry Genomatica, Inc., 4757 Nexus Center Drive, 92121, San Diego, CA, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/3(2015-03-01), 349-360 1367-5435 42:3<349 2015 42 10295