caa a22 4500 606212450 CHVBK 20210128101043.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10295-015-1585-x doi (NATIONALLICENCE)springer-10.1007/s10295-015-1585-x Antoniewicz Maciek Department of Chemical and Biomolecular Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware, 150 Academy St, 19716, Newark, DE, USA aut Methods and advances in metabolic flux analysis: a mini-review [Elektronische Daten] [Maciek Antoniewicz] Metabolic flux analysis (MFA) is one of the pillars of metabolic engineering. Over the past three decades, it has been widely used to quantify intracellular metabolic fluxes in both native (wild type) and engineered biological systems. Through MFA, changes in metabolic pathway fluxes are quantified that result from genetic and/or environmental interventions. This information, in turn, provides insights into the regulation of metabolic pathways and may suggest new targets for further metabolic engineering of the strains. In this mini-review, we discuss and classify the various methods of MFA that have been developed, which include stoichiometric MFA, 13C metabolic flux analysis, isotopic non-stationary 13C metabolic flux analysis, dynamic metabolic flux analysis, and 13C dynamic metabolic flux analysis. For each method, we discuss key advantages and limitations and conclude by highlighting important recent advances in flux analysis approaches. Society for Industrial Microbiology and Biotechnology, 2015 Metabolism nationallicence Isotopic labeling nationallicence Metabolic network model nationallicence Flux quantification nationallicence MFA : Metabolic flux analysis nationallicence DMFA : Dynamic metabolic flux analysis nationallicence 13C-MFA : 13C-metabolic flux analysis nationallicence 13C-DMFA : 13C dynamic metabolic flux analysis nationallicence 13C-NMFA : Isotopic non-stationary 13C-metabolic flux analysis nationallicence SSR : Variance-weighted sum of squared residuals nationallicence Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/3(2015-03-01), 317-325 1367-5435 42:3<317 2015 42 10295 https://doi.org/10.1007/s10295-015-1585-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1585-x text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Antoniewicz Maciek Department of Chemical and Biomolecular Engineering, Metabolic Engineering and Systems Biology Laboratory, University of Delaware, 150 Academy St, 19716, Newark, DE, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/3(2015-03-01), 317-325 1367-5435 42:3<317 2015 42 10295