caa a22 4500 606212477 CHVBK 20210128101043.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10295-014-1518-0 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1518-0 Recent advances in biosynthesis of fatty acids derived products in Saccharomyces cerevisiae via enhanced supply of precursor metabolites [Elektronische Daten] [Jiazhang Lian, Huimin Zhao] Fatty acids or their activated forms, fatty acyl-CoAs and fatty acyl-ACPs, are important precursors to synthesize a wide variety of fuels and chemicals, including but not limited to free fatty acids (FFAs), fatty alcohols (FALs), fatty acid ethyl esters (FAEEs), and alkanes. However, Saccharomyces cerevisiae, an important cell factory, does not naturally accumulate fatty acids in large quantities. Therefore, metabolic engineering strategies were carried out to increase the glycolytic fluxes to fatty acid biosynthesis in yeast, specifically to enhance the supply of precursors, eliminate competing pathways, and bypass the host regulatory network. This review will focus on the genetic manipulation of both structural and regulatory genes in each step for fatty acids overproduction in S. cerevisiae, including from sugar to acetyl-CoA, from acetyl-CoA to malonyl-CoA, and from malonyl-CoA to fatty acyl-CoAs. The downstream pathways for the conversion of fatty acyl-CoAs to the desired products will also be discussed. Society for Industrial Microbiology and Biotechnology, 2014 Fatty acids nationallicence Acetyl-CoA nationallicence Malonyl-CoA nationallicence Fatty acyl-CoAs nationallicence Saccharomyces cerevisiae nationallicence Lian Jiazhang Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA aut Zhao Huimin Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/3(2015-03-01), 437-451 1367-5435 42:3<437 2015 42 10295 https://doi.org/10.1007/s10295-014-1518-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1518-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lian Jiazhang Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Zhao Huimin Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/3(2015-03-01), 437-451 1367-5435 42:3<437 2015 42 10295