caa a22 4500 606212531 CHVBK 20210128101043.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10295-014-1569-2 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1569-2 Engineering the supply chain for protein production/secretion in yeasts and mammalian cells [Elektronische Daten] [Tobias Klein, Jens Niklas, Elmar Heinzle] Metabolic bottlenecks play an increasing role in yeasts and mammalian cells applied for high-performance production of proteins, particularly of pharmaceutical ones that require complex posttranslational modifications. We review the present status and developments focusing on the rational metabolic engineering of such cells to optimize the supply chain for building blocks and energy. Methods comprise selection of beneficial genetic modifications, rational design of media and feeding strategies. Design of better producer cells based on whole genome-wide metabolic network analysis becomes increasingly possible. High-resolution methods of metabolic flux analysis for the complex networks in these compartmented cells are increasingly available.We discuss phenomena that are common to both types of organisms but also those that are different with respect to the supply chain for the production and secretion of pharmaceutical proteins. Society for Industrial Microbiology and Biotechnology, 2015 Klein Tobias Research Area Biochemical Engineering, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorfer Strasse 1a, 1060, Vienna, Austria aut Niklas Jens Genedata AG, Margarethenstrasse 38, 4053, Basel, Switzerland aut Heinzle Elmar Biochemical Engineering Institute, Saarland University, Campus A1.5, 66123, Saarbrücken, Germany aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/3(2015-03-01), 453-464 1367-5435 42:3<453 2015 42 10295 https://doi.org/10.1007/s10295-014-1569-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1569-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Klein Tobias Research Area Biochemical Engineering, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorfer Strasse 1a, 1060, Vienna, Austria aut NATIONALLICENCE 700 1- Niklas Jens Genedata AG, Margarethenstrasse 38, 4053, Basel, Switzerland aut NATIONALLICENCE 700 1- Heinzle Elmar Biochemical Engineering Institute, Saarland University, Campus A1.5, 66123, Saarbrücken, Germany aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/3(2015-03-01), 453-464 1367-5435 42:3<453 2015 42 10295