caa a22 4500 606212574 CHVBK 20210128101043.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10295-015-1669-7 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1669-7 Efficient aspartic acid production by a psychrophile-based simple biocatalyst [Elektronische Daten] [Takahisa Tajima, Mai Hamada, Yutaka Nakashimada, Junichi Kato] We previously constructed a Psychrophile-based Simple bioCatalyst (PSCat) reaction system, in which psychrophilic metabolic enzymes are inactivated by heat treatment, and used it here to study the conversion of aspartic acid from fumaric acid mediated by the activity of aspartate ammonia-lyase (aspartase). In Escherichia coli, the biosynthesis of aspartic acid competes with that of L-malic acid produced from fumaric acid by fumarase. In this study, E. coli aspartase was expressed in psychrophilic Shewanella livingstonensis Ac10 heat treated at 50°C for 15min. The resultant PSCat could convert fumaric acid to aspartic acid without the formation of L-malic acid because of heat inactivation of psychrophilic fumarase activity. Furthermore, alginate-immobilized PSCat produced high yields of aspartic acid and could be re-used nine times. The results of our study suggest that PSCat can be applied in biotechnological production as a new approach to increase the yield of target compounds. Society for Industrial Microbiology and Biotechnology, 2015 Alginate nationallicence Biomass nationallicence Aspartic acid nationallicence Psychrophilic bacteria nationallicence Immobilization nationallicence Tajima Takahisa Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1, Kagamiyama, 739-8530, Higashi-Hiroshima, Japan aut Hamada Mai Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1, Kagamiyama, 739-8530, Higashi-Hiroshima, Japan aut Nakashimada Yutaka Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1, Kagamiyama, 739-8530, Higashi-Hiroshima, Japan aut Kato Junichi Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1, Kagamiyama, 739-8530, Higashi-Hiroshima, Japan aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/10(2015-10-01), 1319-1324 1367-5435 42:10<1319 2015 42 10295 https://doi.org/10.1007/s10295-015-1669-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1669-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tajima Takahisa Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1, Kagamiyama, 739-8530, Higashi-Hiroshima, Japan aut NATIONALLICENCE 700 1- Hamada Mai Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1, Kagamiyama, 739-8530, Higashi-Hiroshima, Japan aut NATIONALLICENCE 700 1- Nakashimada Yutaka Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1, Kagamiyama, 739-8530, Higashi-Hiroshima, Japan aut NATIONALLICENCE 700 1- Kato Junichi Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1, Kagamiyama, 739-8530, Higashi-Hiroshima, Japan aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/10(2015-10-01), 1319-1324 1367-5435 42:10<1319 2015 42 10295