caa a22 4500 606212582 CHVBK 20210128101043.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10295-015-1670-1 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1670-1 Bioprocess for efficient production of recombinant Pichia anomala phytase and its applicability in dephytinizing chick feed and whole wheat flat Indian breads [Elektronische Daten] [Swati Joshi, T. Satyanarayana] The phytase of the yeast Pichia anomala (PPHY) is a suitable biocatalyst as a food and feed additive because of its adequate thermostability, acid stability, protease insensitivity and broad substrate spectrum. The cell-bound nature and low phytase titres are the main bottlenecks for its utility in food and feed industries. In this investigation, we have overcome the problems by constitutive secretory expression of PPHY under glyceraldehyde phosphate dehydrogenase (GAP) promoter. A ~44-fold increase in rPPHY titre has been achieved after optimization of cultural variables by one-variable-at-a-time approach and two factorial statistical design. The use of GAP promoter makes the cultivation of the recombinant P. pastoris straight forward and eliminates the requirement of methanol for induction and hazards associated with its storage. Among metal-phytate complexes, Ca2+ phytate is hydrolyzed more efficiently by rPPHY than Co2+, Mn2+, Mg2+, Fe3+ and Zn2+ phytates. The enzyme is effective in dephytinizing whole wheat unleavened flat Indian breads (naan and tandoori) and different broiler feeds, thus mitigating anti-nutritional effects of phytates. Society for Industrial Microbiology and Biotechnology, 2015 Pichia anomala nationallicence Recombinant phytase nationallicence Anti-nutrient factor nationallicence Dephytinization nationallicence Unleavened flat wheat breads nationallicence Joshi Swati Department of Microbiology, University of Delhi South Campus, 110021, New Delhi, India aut Satyanarayana T. Department of Microbiology, University of Delhi South Campus, 110021, New Delhi, India aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/10(2015-10-01), 1389-1400 1367-5435 42:10<1389 2015 42 10295 https://doi.org/10.1007/s10295-015-1670-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1670-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Joshi Swati Department of Microbiology, University of Delhi South Campus, 110021, New Delhi, India aut NATIONALLICENCE 700 1- Satyanarayana T. Department of Microbiology, University of Delhi South Campus, 110021, New Delhi, India aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/10(2015-10-01), 1389-1400 1367-5435 42:10<1389 2015 42 10295