caa a22 4500 606212590 CHVBK 20210128101043.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10295-015-1658-x doi (NATIONALLICENCE)springer-10.1007/s10295-015-1658-x Towards the commercialization of Botryococcus braunii for triterpenoid production [Elektronische Daten] [Khalid Al-Hothaly, Eric Adetutu, Brian May, Mohamed Taha, Andrew Ball] Botryococcus braunii can accumulate unusually high levels of triterpenoid hydrocarbons making it a potential source of high value chemicals. However, its commercial application is hampered by its slow growth and lack of large-scale studies of triterpenoid hydrocarbon production. This study investigated hydrocarbon production in two race B of B. braunii strains, Overjuyo-3 and Kossou-4, at 25°C in 500L open tanks under artificial lighting in modified BG11 medium over 60days. Maximum growth was reached by 40days with Overjuyo-3 producing more biomass (3.05g L−1) than Kossou-4 (2.55g L−1). However, Kossou-4 produced more oil (0.75g L−1) and triterpenoid hydrocarbons (C30-C34; 50% of oil weight) compared to 0.63g L−1 of oil in Overjuyo-3 with triterpenoid hydrocarbons making up 29% of oil weight. This research demonstrates for the first time that large-scale production of high value triterpenoid hydrocarbon for commercial application is feasible with Kossou-4 strain. Society for Industrial Microbiology and Biotechnology, 2015 Triterpenoid hydrocarbon nationallicence Race B nationallicence Large scale nationallicence Botryococcus braunii nationallicence Biomass nationallicence Al-Hothaly Khalid School of Applied Science, Centre for Environmental Sustainability and Remediation, RMIT University, 3083, Bundoora, VIC, Australia aut Adetutu Eric School of Applied Science, Centre for Environmental Sustainability and Remediation, RMIT University, 3083, Bundoora, VIC, Australia aut May Brian School of Health Sciences, RMIT University, Bundoora, VIC, Australia aut Taha Mohamed School of Applied Science, Centre for Environmental Sustainability and Remediation, RMIT University, 3083, Bundoora, VIC, Australia aut Ball Andrew School of Applied Science, Centre for Environmental Sustainability and Remediation, RMIT University, 3083, Bundoora, VIC, Australia aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/10(2015-10-01), 1415-1418 1367-5435 42:10<1415 2015 42 10295 https://doi.org/10.1007/s10295-015-1658-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1658-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Al-Hothaly Khalid School of Applied Science, Centre for Environmental Sustainability and Remediation, RMIT University, 3083, Bundoora, VIC, Australia aut NATIONALLICENCE 700 1- Adetutu Eric School of Applied Science, Centre for Environmental Sustainability and Remediation, RMIT University, 3083, Bundoora, VIC, Australia aut NATIONALLICENCE 700 1- May Brian School of Health Sciences, RMIT University, Bundoora, VIC, Australia aut NATIONALLICENCE 700 1- Taha Mohamed School of Applied Science, Centre for Environmental Sustainability and Remediation, RMIT University, 3083, Bundoora, VIC, Australia aut NATIONALLICENCE 700 1- Ball Andrew School of Applied Science, Centre for Environmental Sustainability and Remediation, RMIT University, 3083, Bundoora, VIC, Australia aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/10(2015-10-01), 1415-1418 1367-5435 42:10<1415 2015 42 10295