caa a22 4500 606212604 CHVBK 20210128101043.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10295-015-1655-0 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1655-0 Yeast Kluyveromyces lactis as host for expression of the bacterial lipase: cloning and adaptation of the new lipase gene from Serratia sp [Elektronische Daten] [Rimantas Šiekštelė, Aušra Veteikytė, Bronius Tvaska, Inga Matijošytė] Many microbial lipases have been successfully expressed in yeasts, but not in industrially attractive Kluyveromyces lactis, which among other benefits can be cultivated on a medium supplemented with whey--cheap and easily available industrial waste. A new bacterial lipase from Serratia sp. was isolated and for the first time expressed into the yeast Kluyveromyces lactis by heterologous protein expression system based on a strong promoter of Kluyveromyces marxianus triosephosphate isomerase gene and signal peptide of Kluyveromyces marxianus endopolygalacturonase gene. In addition, the bacterial lipase gene was synthesized de novo by taking into account a codon usage bias optimal for K. lactis and was expressed into the yeast K. lactis also. Both resulting strains were characterized by high output level of the target protein secreted extracellularly. Secreted lipases were characterized for activity and stability. Society for Industrial Microbiology and Biotechnology, 2015 Lipase nationallicence Serratia sp nationallicence de novo synthesis nationallicence Kluyveromyces lactis nationallicence Whey nationallicence Šiekštelė Rimantas Sector of Applied Biocatalysis, Institute of Biotechnology, Vilnius University, V.A. Graičiūno str. 8, 02241, Vilnius, Lithuania aut Veteikytė Aušra Sector of Applied Biocatalysis, Institute of Biotechnology, Vilnius University, V.A. Graičiūno str. 8, 02241, Vilnius, Lithuania aut Tvaska Bronius JSC Biocentras, V.A. Graičiūno str. 10, 02241, Vilnius, Lithuania aut Matijošytė Inga Sector of Applied Biocatalysis, Institute of Biotechnology, Vilnius University, V.A. Graičiūno str. 8, 02241, Vilnius, Lithuania aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/10(2015-10-01), 1309-1317 1367-5435 42:10<1309 2015 42 10295 https://doi.org/10.1007/s10295-015-1655-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1655-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Šiekštelė Rimantas Sector of Applied Biocatalysis, Institute of Biotechnology, Vilnius University, V.A. Graičiūno str. 8, 02241, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Veteikytė Aušra Sector of Applied Biocatalysis, Institute of Biotechnology, Vilnius University, V.A. Graičiūno str. 8, 02241, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Tvaska Bronius JSC Biocentras, V.A. Graičiūno str. 10, 02241, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Matijošytė Inga Sector of Applied Biocatalysis, Institute of Biotechnology, Vilnius University, V.A. Graičiūno str. 8, 02241, Vilnius, Lithuania aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/10(2015-10-01), 1309-1317 1367-5435 42:10<1309 2015 42 10295