caa a22 4500 606212620 CHVBK 20210128101043.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10295-015-1673-y doi (NATIONALLICENCE)springer-10.1007/s10295-015-1673-y Expression of plectasin in Bacillus subtilis using SUMO technology by a maltose-inducible vector [Elektronische Daten] [Licong Zhang, Xiaodan Li, Dandan Wei, Jue Wang, Anshan Shan, Zhongyu Li] Plectasin, the first fungus defensin, is especially efficient against Gram-positive bacteria. To explore an effective approach for expressing plectasin in Bacillus subtilis, the sequence encoding plectasin fused with the small ubiquitin-like modifier (SUMO) gene, the 6×His gene and the signal peptide of SacB were cloned into an E. coli-B. subtilis shuttle vector pGJ148 in which the maltose utilization operon promoter Pglv directed the expression. The fusion protein successfully secreted in culture and approximately, 41mg of the recombinant fusion protein SUMO-plectasin was purified per liter of culture supernatant. After purification by Ni-NTA resin column and digestion by SUMO protease, 5.5mg of plectasin with a purity of 94% was obtained from 1L fermentation culture. Recombinant plectasin was found inhibition activity against S. pneumoniae, S. aureus and S. epidermidis. These results indicate that the maltose-induced expression system may be a safe and efficient way for the large-scale production of soluble peptides in B. subtilis. Society for Industrial Microbiology and Biotechnology, 2015 Antimicrobial peptide nationallicence Plectasin nationallicence Bacillus subtilis nationallicence Maltose-inducible promoter nationallicence SUMO nationallicence Zhang Licong Institute of Animal Nutrition, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, China aut Li Xiaodan Institute of Animal Nutrition, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, China aut Wei Dandan Institute of Animal Nutrition, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, China aut Wang Jue Institute of Animal Nutrition, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, China aut Shan Anshan Institute of Animal Nutrition, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, China aut Li Zhongyu Institute of Animal Nutrition, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/10(2015-10-01), 1369-1376 1367-5435 42:10<1369 2015 42 10295 https://doi.org/10.1007/s10295-015-1673-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1673-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Licong Institute of Animal Nutrition, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, China aut NATIONALLICENCE 700 1- Li Xiaodan Institute of Animal Nutrition, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, China aut NATIONALLICENCE 700 1- Wei Dandan Institute of Animal Nutrition, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, China aut NATIONALLICENCE 700 1- Wang Jue Institute of Animal Nutrition, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, China aut NATIONALLICENCE 700 1- Shan Anshan Institute of Animal Nutrition, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, China aut NATIONALLICENCE 700 1- Li Zhongyu Institute of Animal Nutrition, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, 150030, Harbin, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/10(2015-10-01), 1369-1376 1367-5435 42:10<1369 2015 42 10295