caa a22 4500 606212639 CHVBK 20210128101043.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10295-015-1648-z doi (NATIONALLICENCE)springer-10.1007/s10295-015-1648-z Enhanced production of ( R , R )-2,3-butanediol by metabolically engineered Klebsiella oxytoca [Elektronische Daten] [Jong Park, Chelladurai Rathnasingh, Hyohak Song] Microbial fermentation produces a racemic mixture of 2,3-butanediol ((R,R)-BD, (S,S)-BD, and meso-BD), and the compositions and physiochemical properties vary from microorganism to microorganism. Although the meso form is much more difficult to transport and store because of its higher freezing point than those of the optically active forms, most microorganisms capable of producing 2,3-BD mainly yield meso-2,3-BD. Thus, we developed a metabolically engineered (R,R)-2,3-BD overproducing strain using a Klebsiella oxytoca ΔldhA ΔpflB strain, which shows an outstanding 2,3-BD production performance with more than 90% of the meso form. A budC gene encoding 2,3-BD dehydrogenase in the K. oxytoca ΔldhA ΔpflB strain was replaced with an exogenous gene encoding (R,R)-2,3-BD dehydrogenase from Paenibacillus polymyxa (K. oxytoca ΔldhA ΔpflB ΔbudC::PBDH strain), and then its expression level was further amplified with using a pBBR1MCS plasmid. The fed-batch fermentation of the K. oxytoca ΔldhA ΔpflB ΔbudC::PBDH (pBBR-PBDH) strain with intermittent glucose feeding allowed the production of 106.7g/L of (R,R)-2,3-BD [meso-2,3-BD, 9.3g/L], with a yield of 0.40g/g and a productivity of 3.1g/L/h, which should be useful for the industrial application of 2,3-BD. Society for Industrial Microbiology and Biotechnology, 2015 meso -2,3-butanediol nationallicence ( R , R )-2,3-butanediol nationallicence Klebsiella oxytoca ΔldhA ΔpflB nationallicence Park Jong Research and Development Center, GS Caltex Corporation, 104-4 Munji-dong, Yuseong-gu, 305-380, Taejon, Republic of Korea aut Rathnasingh Chelladurai Research and Development Center, GS Caltex Corporation, 104-4 Munji-dong, Yuseong-gu, 305-380, Taejon, Republic of Korea aut Song Hyohak Research and Development Center, GS Caltex Corporation, 104-4 Munji-dong, Yuseong-gu, 305-380, Taejon, Republic of Korea aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/10(2015-10-01), 1419-1425 1367-5435 42:10<1419 2015 42 10295 https://doi.org/10.1007/s10295-015-1648-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1648-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park Jong Research and Development Center, GS Caltex Corporation, 104-4 Munji-dong, Yuseong-gu, 305-380, Taejon, Republic of Korea aut NATIONALLICENCE 700 1- Rathnasingh Chelladurai Research and Development Center, GS Caltex Corporation, 104-4 Munji-dong, Yuseong-gu, 305-380, Taejon, Republic of Korea aut NATIONALLICENCE 700 1- Song Hyohak Research and Development Center, GS Caltex Corporation, 104-4 Munji-dong, Yuseong-gu, 305-380, Taejon, Republic of Korea aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/10(2015-10-01), 1419-1425 1367-5435 42:10<1419 2015 42 10295