caa a22 4500 606212671 CHVBK 20210128101043.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10295-014-1536-y doi (NATIONALLICENCE)springer-10.1007/s10295-014-1536-y Redirecting metabolic flux in Saccharomyces cerevisiae through regulation of cofactors in UMP production [Elektronische Daten] [Yong Chen, Qingguo Liu, Xiaochun Chen, Jinglan Wu, Ting Guo, Chenjie Zhu, Hanjie Ying] Although it is generally known that cofactors play a major role in the production of different fermentation products, their role has not been thoroughly and systematically studied. To understand the impact of cofactors on physiological functions, a systematic approach was applied, which involved redox state analysis, energy charge analysis, and metabolite analysis. Using uridine 5′-monophosphate metabolism in Saccharomyces cerevisiae as a model, we demonstrated that regulation of intracellular the ratio of NADPH to NADP+ not only redistributed the carbon flux between the glycolytic and pentose phosphate pathways, but also regulated the redox state of NAD(H), resulting in a significant change of ATP, and a significantly altered spectrum of metabolic products. Society for Industrial Microbiology and Biotechnology, 2015 Cofactor nationallicence Uridine 5′-monophosphate nationallicence Redox state analysis energy nationallicence Charge analysis nationallicence Metabolite analysis nationallicence Chen Yong College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, 210009, Nanjing, People's Republic of China aut Liu Qingguo College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, 210009, Nanjing, People's Republic of China aut Chen Xiaochun College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, 210009, Nanjing, People's Republic of China aut Wu Jinglan College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, 210009, Nanjing, People's Republic of China aut Guo Ting College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, 210009, Nanjing, People's Republic of China aut Zhu Chenjie College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, 210009, Nanjing, People's Republic of China aut Ying Hanjie College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, 210009, Nanjing, People's Republic of China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 577-583 1367-5435 42:4<577 2015 42 10295 https://doi.org/10.1007/s10295-014-1536-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1536-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Yong College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, 210009, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Liu Qingguo College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, 210009, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Chen Xiaochun College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, 210009, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Wu Jinglan College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, 210009, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Guo Ting College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, 210009, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Chenjie College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, 210009, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Ying Hanjie College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, 210009, Nanjing, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 577-583 1367-5435 42:4<577 2015 42 10295