caa a22 4500 606212698 CHVBK 20210128101044.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10295-015-1588-7 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1588-7 Quantifying the sensitivity of G. oxydans ATCC621H and DSM 3504 to osmotic stress triggered by soluble buffers [Elektronische Daten] [B. Luchterhand, T. Fischöder, A. Grimm, S. Wewetzer, M. Wunderlich, T. Schlepütz, J. Büchs] In Gluconobacter oxydans cultivations on glucose, CaCO3 is typically used as pH-buffer. This buffer, however, has disadvantages: suspended CaCO3 particles make the medium turbid, thereby, obstructing analysis of microbial growth via optical density and scattered light. Upon searching for alternative soluble pH-buffers, bacterial growth and productivity was inhibited most probably due to osmotic stress. Thus, this study investigates in detail the osmotic sensitivity of G. oxydans ATCC621H and DSM3504 using the Respiratory Activity MOnitoring System. The tested soluble pH-buffers and other salts attained osmolalities of 0.32-1.19osmolkg−1. This study shows that G. oxydans ATCC 621H and DSM 3504 respond quite sensitively to increased osmolality in comparison to other microbial strains of industrial interest. Osmolality values of >0.5osmolkg−1 should not be exceeded to avoid inhibition of growth and product formation. This osmolality threshold needs to be considered when working with soluble pH-buffers. Society for Industrial Microbiology and Biotechnology, 2015 Gluconobacter oxydans nationallicence pH-buffer systems nationallicence Water activity nationallicence Osmotic pressure nationallicence RAMOS nationallicence Luchterhand B. AVT, Chair of Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany aut Fischöder T. Department of Biotechnology/Biomaterial Sciences and Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Pauwelsstr. 20, 52074, Aachen, Germany aut Grimm A. Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany aut Wewetzer S. AVT, Chair of Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany aut Wunderlich M. AVT, Chair of Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany aut Schlepütz T. AVT, Chair of Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany aut Büchs J. AVT, Chair of Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 585-600 1367-5435 42:4<585 2015 42 10295 https://doi.org/10.1007/s10295-015-1588-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1588-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luchterhand B. AVT, Chair of Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Fischöder T. Department of Biotechnology/Biomaterial Sciences and Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Pauwelsstr. 20, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Grimm A. Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Wewetzer S. AVT, Chair of Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Wunderlich M. AVT, Chair of Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Schlepütz T. AVT, Chair of Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Büchs J. AVT, Chair of Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 585-600 1367-5435 42:4<585 2015 42 10295