caa a22 4500 606212701 CHVBK 20210128101044.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10295-014-1579-0 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1579-0 Comparative transcriptomic analysis reveals novel genes and regulatory mechanisms of Tetragenococcus halophilus in response to salt stress [Elektronische Daten] [Licui Liu, Lifang Si, Xin Meng, Lixin Luo] Tetragenococcus halophilus, a moderately halophilic Gram-positive bacterium, was isolated from Chinese style soy sauce. This species is a valuable resource for investigating salt tolerance mechanisms and improving salinity resistance in microorganisms. RNA-seq was used to sequence T. halophilus samples treated with 0M (T1), 1M (T2), and 3.5M NaCl (T3). Comparative transcriptomic analyses of the different treatments were performed using gene ontology and Kyoto encyclopedia of genes and genome. The comparison of T1 and T2 by RNA-seq revealed that genes involved in transcription, translation, membrane system, and division were highly up-regulated under optimum salt condition. The comparison of T2 and T3 showed that genes related to heat shock proteins or the ATP-binding cassette transport systems were significantly up-regulated under maximum-salt condition. In addition, a considerable proportion of the significantly differently expressed genes identified in this study are novel. These data provide a crucial resource that may determine specific responses to salt stress in T. halophilus. Society for Industrial Microbiology and Biotechnology, 2015 Tetragenococcus halophilus nationallicence Transcriptomic nationallicence Salt stress nationallicence RNA sequencing nationallicence Regulatory mechanisms nationallicence Liu Licui School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China aut Si Lifang School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China aut Meng Xin School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China aut Luo Lixin School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 601-616 1367-5435 42:4<601 2015 42 10295 https://doi.org/10.1007/s10295-014-1579-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1579-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Licui School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China aut NATIONALLICENCE 700 1- Si Lifang School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China aut NATIONALLICENCE 700 1- Meng Xin School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China aut NATIONALLICENCE 700 1- Luo Lixin School of Bioscience and Bioengineering, South China University of Technology, 510006, Guangzhou, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 601-616 1367-5435 42:4<601 2015 42 10295