caa a22 4500 60621271X CHVBK 20210128101044.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10295-015-1584-y doi (NATIONALLICENCE)springer-10.1007/s10295-015-1584-y Identification of novel genes responsible for salt tolerance by transposon mutagenesis in Saccharomyces cerevisiae [Elektronische Daten] [Won-Kun Park, Ji-Won Yang, Hyun-Soo Kim] Saccharomyces cerevisiae strains tolerant to salt stress are important for the production of single-cell protein using kimchi waste brine. In this study, two strains (TN-1 and TN-2) tolerant of up to 10% (w/v) NaCl were isolated by screening a transposon-mediated mutant library. The determination of transposon insertion sites and Northern blot analysis identified two genes, MDJ1 and VPS74, and revealed disruptions of the open reading frame of both genes, indicating that salt tolerance can be conferred. Such tolerant phenotypes reverted to sensitive phenotypes on the autologous or overexpression of each gene. The two transposon mutants grew faster than the control strain when cultured at 30°C in rich medium containing 5, 7.5 or 10% NaCl. The genes identified in this study may provide a basis for application in developing industrial yeast strains. Society for Industrial Microbiology and Biotechnology, 2015 Yeast nationallicence Transposon nationallicence Salt tolerance nationallicence Park Won-Kun Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 350-701, Daejeon, Korea aut Yang Ji-Won Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 350-701, Daejeon, Korea aut Kim Hyun-Soo Department of Food Science and Industry, Jungwon University, 85, Munmu-ro, Goesan-eup, Goesan-gun, 367-805, Chungbuk, Korea aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 567-575 1367-5435 42:4<567 2015 42 10295 https://doi.org/10.1007/s10295-015-1584-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1584-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park Won-Kun Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 350-701, Daejeon, Korea aut NATIONALLICENCE 700 1- Yang Ji-Won Department of Chemical and Biomolecular Engineering, KAIST, 291, Daehak-ro, Yuseong-gu, 350-701, Daejeon, Korea aut NATIONALLICENCE 700 1- Kim Hyun-Soo Department of Food Science and Industry, Jungwon University, 85, Munmu-ro, Goesan-eup, Goesan-gun, 367-805, Chungbuk, Korea aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 567-575 1367-5435 42:4<567 2015 42 10295