caa a22 4500 606212728 CHVBK 20210128101044.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10295-014-1573-6 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1573-6 Engineering the robustness of Saccharomyces cerevisiae by introducing bifunctional glutathione synthase gene [Elektronische Daten] [Zhiqi Qiu, Zujun Deng, Hongming Tan, Shining Zhou, Lixiang Cao] Robust, high-yielding Saccharomyces cerevisiae is highly desirable for cost-effective cellulosic ethanol production. In this study, the bifunctional glutathione (GSH) synthetase genes GCSGS at high copy number was integrated into ribosomal DNA of S. cerevisiae by Cre-LoxP system. Threefold higher GSH contents (54.9μmol/g dry weight) accumulated in the engineered strain BY-G compared to the reference strain. Tolerance of BY-G to H2O2 (3mM), temperature (40°C), furfural (10mM), hydroxymethylfurfural (HMF, 10mM) and 0.5mM Cd2+ increased compared to reference strain. Twofold higher ethanol concentration was obtained by BY-G in simultaneous saccharification and fermentation of corn stover compared to the reference strain. The results showed that intracellular GSH content of S. cerevisiae has an influence on robustness. The strategy is used to engineer S. cerevisiae strains adaptive to a combination of tolerance to inhibitors and raised temperature that may occur in high solid simultaneous saccharification and fermentation of lignocellulosic feedstocks. Society for Industrial Microbiology and Biotechnology, 2014 Bioethanol nationallicence GSH nationallicence Lignocellulose nationallicence S. cerevisiae nationallicence Robustness nationallicence Qiu Zhiqi School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Deng Zujun Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, School of Basic Courses, Guangdong Pharmaceutical University, 510006, Guangzhou, People's Republic of China aut Tan Hongming School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Zhou Shining School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Cao Lixiang School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 537-542 1367-5435 42:4<537 2015 42 10295 https://doi.org/10.1007/s10295-014-1573-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1573-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Qiu Zhiqi School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Deng Zujun Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, School of Basic Courses, Guangdong Pharmaceutical University, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Tan Hongming School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Shining School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Cao Lixiang School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 537-542 1367-5435 42:4<537 2015 42 10295