caa a22 4500 606212736 CHVBK 20210128101044.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10295-014-1565-6 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1565-6 Metabolic engineering of Escherichia coli to produce zeaxanthin [Elektronische Daten] [Xi-Ran Li, Gui-Qiao Tian, Hong-Jie Shen, Jian-Zhong Liu] Zeaxanthin is a high-value carotenoid that is used in nutraceuticals, cosmetics, food, and animal feed industries. Zeaxanthin is chemically synthesized or purified from microorganisms as a natural product; however, increasing demand requires development of alternative sources such as heterologous biosynthesis by recombinant bacteria. For this purpose, we molecularly engineered Escherichia coli to optimize the synthesis of zeaxanthin from lycopene using fusion protein-mediated substrate channeling as well as by the introduction of tunable intergenic regions. The tunable intergenic regions approach was more efficient compared with protein fusion for coordinating expression of lycopene β-cyclase gene crtY and β-carotene 3-hydroxylase gene crtZ. The influence of the substrate channeling effect suggests that the reaction catalyzed by CrtZ is the rate-limiting step in zeaxanthin biosynthesis. Then Pantoea ananatis, Pantoea agglomerans and Haematococcus pluvialis crtZ were compared. Because P. ananatis crtZ is superior to that of P. agglomerans or H. pluvialis for zeaxanthin production, we used it to generate a recombinant strain of E. coli BETA-1 containing pZSPBA-2(P37-crtZPAN) that produced higher amounts of zeaxanthin (11.95±0.21mg/g dry cell weight) than other engineered E. coli strains described in the literature. Society for Industrial Microbiology and Biotechnology, 2014 Zeaxanthin nationallicence Escherichia coli nationallicence Metabolic engineering nationallicence Substrate channeling nationallicence Library of tunable intergenic regions nationallicence Li Xi-Ran Biotechnology Research Center and Biomedical Center, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Tian Gui-Qiao Biotechnology Research Center and Biomedical Center, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Shen Hong-Jie Biotechnology Research Center and Biomedical Center, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Liu Jian-Zhong Biotechnology Research Center and Biomedical Center, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 627-636 1367-5435 42:4<627 2015 42 10295 https://doi.org/10.1007/s10295-014-1565-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1565-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Xi-Ran Biotechnology Research Center and Biomedical Center, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Tian Gui-Qiao Biotechnology Research Center and Biomedical Center, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Shen Hong-Jie Biotechnology Research Center and Biomedical Center, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liu Jian-Zhong Biotechnology Research Center and Biomedical Center, School of Life Sciences, Sun Yat-sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 627-636 1367-5435 42:4<627 2015 42 10295