caa a22 4500 606212744 CHVBK 20210128101044.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10295-015-1586-9 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1586-9 Immobilization of Pseudomonas fluorescens lipase on hydrophobic supports and application in biodiesel synthesis by transesterification of vegetable oils in solvent-free systems [Elektronische Daten] [Lionete Lima, Gladson Oliveira, Mayerlenis Rojas, Heizir Castro, Patrícia Da Rós, Adriano Mendes, Raquel Giordano, Paulo Tardioli] This work describes the preparation of biocatalysts for ethanolysis of soybean and babassu oils in solvent-free systems. Polystyrene, Amberlite (XAD-7HP), and octyl-silica were tested as supports for the immobilization of Pseudomonas fluorescens lipase (PFL). The use of octyl-silica resulted in a biocatalyst with high values of hydrolytic activity (650.0±15.5IU/g), immobilization yield (91.3±0.3%), and recovered activity (82.1±1.5%). PFL immobilized on octyl-silica was around 12-fold more stable than soluble PFL, at 45°C and pH 8.0, in the presence of ethanol at 36% (v/v). The biocatalyst provided high vegetable oil transesterification yields of around 97.5% after 24h of reaction using babassu oil and around 80% after 48h of reaction using soybean oil. The PFL-octyl-silica biocatalyst retained around 90% of its initial activity after five cycles of transesterification of soybean oil. Octyl-silica is a promising support that can be used to immobilize PFL for subsequent application in biodiesel synthesis. Society for Industrial Microbiology and Biotechnology, 2015 Lipase nationallicence Hydrophobic adsorption nationallicence Octyl-silica nationallicence Biodiesel nationallicence Soy and babassu oils nationallicence Lima Lionete Graduate Program in Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil aut Oliveira Gladson Graduate Program in Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil aut Rojas Mayerlenis Graduate Program in Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil aut Castro Heizir Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, Lorena, Brazil aut Da Rós Patrícia Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, Lorena, Brazil aut Mendes Adriano Institute of Chemistry, Federal University of Alfenas, Alfenas, Brazil aut Giordano Raquel Graduate Program in Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil aut Tardioli Paulo Graduate Program in Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 523-535 1367-5435 42:4<523 2015 42 10295 https://doi.org/10.1007/s10295-015-1586-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1586-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lima Lionete Graduate Program in Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Oliveira Gladson Graduate Program in Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Rojas Mayerlenis Graduate Program in Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Castro Heizir Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, Lorena, Brazil aut NATIONALLICENCE 700 1- Da Rós Patrícia Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, Lorena, Brazil aut NATIONALLICENCE 700 1- Mendes Adriano Institute of Chemistry, Federal University of Alfenas, Alfenas, Brazil aut NATIONALLICENCE 700 1- Giordano Raquel Graduate Program in Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Tardioli Paulo Graduate Program in Chemical Engineering, Federal University of São Carlos, Rodovia Washington Luiz, km 235, 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 523-535 1367-5435 42:4<523 2015 42 10295