caa a22 4500 606212779 CHVBK 20210128101044.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10295-014-1574-5 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1574-5 Carbon-limited fed-batch production of medium-chain-length polyhydroxyalkanoates by a phaZ -knockout strain of Pseudomonas putida KT2440 [Elektronische Daten] [Minh Vo, Kenton Ko, Bruce Ramsay] A medium-chain-length poly-3-hydroxyalkanote (MCL-PHA) depolymerase knockout mutant of Pseudomonas putida KT2440 was produced by double homologous recombination. A carbon-limited shake-flask study confirmed that depolymerase activity was eliminated. Lysis of both mutant and wild-type strains occurred under these conditions. In carbon-limited, fed-batch culture, the yield of unsaturated monomers from unsaturated substrate averaged only 0.62molmol−1 for the phaZ minus strain compared to 0.72molmol−1 for the wild type. The mutant strain also produced more CO2 and less residual biomass from the same amount of carbon substrate. However, most results indicated that elimination of PHA depolymerase activity had little impact on the overall yield of biomass and PHA. Society for Industrial Microbiology and Biotechnology, 2015 phaZ nationallicence Knockout nationallicence PHA nationallicence putida nationallicence Depolymerase nationallicence Vo Minh Chemical Engineering, Queen's University, K7L 3N6, Kingston, ON, Canada aut Ko Kenton Biology, Queen's University, 116 Barrie St., K7L 3N6, Kingston, ON, Canada aut Ramsay Bruce Chemical Engineering, Queen's University, K7L 3N6, Kingston, ON, Canada aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 637-646 1367-5435 42:4<637 2015 42 10295 https://doi.org/10.1007/s10295-014-1574-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1574-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vo Minh Chemical Engineering, Queen's University, K7L 3N6, Kingston, ON, Canada aut NATIONALLICENCE 700 1- Ko Kenton Biology, Queen's University, 116 Barrie St., K7L 3N6, Kingston, ON, Canada aut NATIONALLICENCE 700 1- Ramsay Bruce Chemical Engineering, Queen's University, K7L 3N6, Kingston, ON, Canada aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 637-646 1367-5435 42:4<637 2015 42 10295