caa a22 4500 606212809 CHVBK 20210128101044.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10295-014-1580-7 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1580-7 Characterization of a novel thermophilic phospholipase B from Thermotoga lettingae TMO: applicability in enzymatic degumming of vegetable oils [Elektronische Daten] [Tao Wei, Chunping Xu, Xuan Yu, Weiwei Jia, Kunpeng Yang, Chunxiao Jia, Duobin Mao] A novel phospholipase B (TLPLB) from Thermotoga lettingae TMO has been cloned, functionally overexpressed in Escherichia coli and purified to homogeneity. Gas chromatography indicated that the enzyme could efficiently hydrolyze both the sn-1 and sn-2 ester bonds of 1-palmitoyl-2-oleoyl phosphatidylcholine as phospholipase B. TLPLB was optimally active at 70°C and pH 5.5, respectively. Its thermostability is relatively high with a half-life of 240min at 90°C. TLPLB also displayed remarkable organic solvent tolerance and maintained approximately 91-161% of its initial activity in 20 and 50% (v/v) hydrophobic organic solvents after incubation for 168h. Furthermore, TLPLB exhibited high degumming activity towards rapeseed, soybean, peanut and sunflower seed oils, where the phosphorus contents were decreased from 225.2, 189.3, 85.6 and 70.4mg/kg to 4.9, 4.7, 3.2 and 2.2mg/kg within 5h, respectively. TLPLB could therefore be used for the degumming of vegetable oils. Society for Industrial Microbiology and Biotechnology, 2015 Phospholipase B nationallicence Thermophilic nationallicence Organic solvent tolerance nationallicence Degumming nationallicence Vegetable oil nationallicence Wei Tao School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, 450002, Zhengzhou, People's Republic of China aut Xu Chunping School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, 450002, Zhengzhou, People's Republic of China aut Yu Xuan School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, 450002, Zhengzhou, People's Republic of China aut Jia Weiwei School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, 450002, Zhengzhou, People's Republic of China aut Yang Kunpeng School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, 450002, Zhengzhou, People's Republic of China aut Jia Chunxiao School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, 450002, Zhengzhou, People's Republic of China aut Mao Duobin School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, 450002, Zhengzhou, People's Republic of China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 515-522 1367-5435 42:4<515 2015 42 10295 https://doi.org/10.1007/s10295-014-1580-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1580-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wei Tao School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, 450002, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Xu Chunping School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, 450002, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yu Xuan School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, 450002, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Jia Weiwei School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, 450002, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yang Kunpeng School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, 450002, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Jia Chunxiao School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, 450002, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Mao Duobin School of Food and Biological Engineering, Zhengzhou University of Light Industry, 5 Dongfeng Rd, 450002, Zhengzhou, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 515-522 1367-5435 42:4<515 2015 42 10295