caa a22 4500 606212825 CHVBK 20210128101044.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10295-014-1582-5 doi (NATIONALLICENCE)springer-10.1007/s10295-014-1582-5 Expression and one-step purification of the antimicrobial peptide cathelicidin-BF using the intein system in Bacillus subtilis [Elektronische Daten] [Qing He, Ai-yun Fu, Tian-jiao Li] The intein expression system has been widely applied in Escherichia coli to express various proteins and peptides. However, the removal of endotoxin from the recombinant proteins expressed in E. coli is very difficult and therefore complicates the purification process. In this study, we constructed an intein-based expression vector for an antimicrobial peptide (cathelicidin from Bungarus fasciatus) and expressed the intein fusion peptide in a Bacillus subtilis expression system. The fusion peptide was secreted into the culture medium, identified by Western blot and purified by affinity chromatography and intein self-cleavage in just one step. Approximately, 0.5mg peptide was obtained from 1 litre of culture medium. The purified peptide showed antimicrobial activity. Our results indicate that the intein expression system may be a safe and efficient method to produce soluble peptides and proteins in B. subtilis. Society for Industrial Microbiology and Biotechnology, 2015 Intein nationallicence Bacillus subtilis nationallicence Cathelicidin nationallicence Protein purification nationallicence He Qing Key University Laboratory of Biotechnology and Utilization of Bio-resource of Shandong, College of Life Science, Dezhou University, 566 University Road West, 253023, Dezhou, Shandong Province, China aut Fu Ai-yun Key University Laboratory of Biotechnology and Utilization of Bio-resource of Shandong, College of Life Science, Dezhou University, 566 University Road West, 253023, Dezhou, Shandong Province, China aut Li Tian-jiao Key University Laboratory of Biotechnology and Utilization of Bio-resource of Shandong, College of Life Science, Dezhou University, 566 University Road West, 253023, Dezhou, Shandong Province, China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 647-653 1367-5435 42:4<647 2015 42 10295 https://doi.org/10.1007/s10295-014-1582-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-014-1582-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- He Qing Key University Laboratory of Biotechnology and Utilization of Bio-resource of Shandong, College of Life Science, Dezhou University, 566 University Road West, 253023, Dezhou, Shandong Province, China aut NATIONALLICENCE 700 1- Fu Ai-yun Key University Laboratory of Biotechnology and Utilization of Bio-resource of Shandong, College of Life Science, Dezhou University, 566 University Road West, 253023, Dezhou, Shandong Province, China aut NATIONALLICENCE 700 1- Li Tian-jiao Key University Laboratory of Biotechnology and Utilization of Bio-resource of Shandong, College of Life Science, Dezhou University, 566 University Road West, 253023, Dezhou, Shandong Province, China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/4(2015-04-01), 647-653 1367-5435 42:4<647 2015 42 10295