caa a22 4500 606212868 CHVBK 20210128101044.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10295-015-1643-4 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1643-4 Isolation and characterization of Paenibacillus polymyxa LY214, a camptothecin-producing endophytic bacterium from Camptotheca acuminata [Elektronische Daten] [Xiang Pu, Fei Chen, Yun Yang, Xixing Qu, Guolin Zhang, Yinggang Luo] Camptothecin (CPT) is mainly produced and extracted from Camptotheca acuminata and Nothapodytes foetida for pharmaceutical use, i.e., the starting material for chemical conversion to the clinical CPT-type drugs. As the third largest plant anticancer drug, the heavy demand on CPT from global market leads to many research efforts to identify new sources for CPT production. Herein we report the isolation and characterization of a CPT-producing endophytic bacterium Paenibacillus polymyxa LY214 from Camptotheca acuminata. A 10.7μgl−1 of CPT was presented in the fermentation broth of P. polymyxa LY214. Its CPT production decreased sharply when the strain of the 2nd generation of P. polymyxa LY214 was cultured and fermented. However, the CPT production remained relatively constant from 2.8μgl−1 of the 2nd generation to 0.8μgl−1 of the 8th generation of P. polymyxa LY214 under optimized fermentation conditions. A 15- to 30-fold increase of CPT yield was observed when the optimized fermentation conditions, together with the addition of putative biosynthetic precursors of CPT and adsorbent resin XAD16, were applied to ferment the strains of the 7th and 8th generation of P. polymyxa LY214. Bioinformatics analysis of the relative species of P. polymyxa LY214 indicates its potential to produce CPT, which will be helpful to decipher the mysteries of CPT biosynthesis. Society for Industrial Microbiology and Biotechnology, 2015 Camptothecin nationallicence Endophytic bacterium nationallicence Paenibacillus polymyxa LY214 nationallicence Adsorbent resin nationallicence Pu Xiang Center for Natural Products Research, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, People's Republic of China aut Chen Fei Center for Natural Products Research, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, People's Republic of China aut Yang Yun Center for Natural Products Research, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, People's Republic of China aut Qu Xixing Center for Natural Products Research, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, People's Republic of China aut Zhang Guolin Center for Natural Products Research, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, People's Republic of China aut Luo Yinggang Center for Natural Products Research, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, People's Republic of China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/8(2015-08-01), 1197-1202 1367-5435 42:8<1197 2015 42 10295 https://doi.org/10.1007/s10295-015-1643-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1643-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pu Xiang Center for Natural Products Research, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Chen Fei Center for Natural Products Research, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Yang Yun Center for Natural Products Research, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Qu Xixing Center for Natural Products Research, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Guolin Center for Natural Products Research, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Luo Yinggang Center for Natural Products Research, Chengdu Institute of Biology, Chinese Academy of Sciences, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/8(2015-08-01), 1197-1202 1367-5435 42:8<1197 2015 42 10295