caa a22 4500 606212884 CHVBK 20210128101044.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10295-015-1638-1 doi (NATIONALLICENCE)springer-10.1007/s10295-015-1638-1 R -acetoin accumulation and dissimilation in Klebsiella pneumoniae [Elektronische Daten] [Dexin Wang, Jidong Zhou, Chuan Chen, Dong Wei, Jiping Shi, Biao Jiang, Pengfu Liu, Jian Hao] Klebsiella pneumoniae is a 2,3-butanediol producer, and R-acetoin is an intermediate of 2,3-butanediol production. R-acetoin accumulation and dissimilation in K. pneumoniae was studied here. A budC mutant, which has lost 2,3-butanediol dehydrogenase activity, accumulated high levels of R-acetoin in culture broth. However, after glucose was exhausted, the accumulated R-acetoin could be reused by the cells as a carbon source. Acetoin dehydrogenase enzyme system, encoded by acoABCD, was responsible for R-acetoin dissimilation. acoABCD mutants lost the ability to grow on acetoin as the sole carbon source, and the acetoin accumulated could not be dissimilated. However, in the presence of another carbon source, the acetoin accumulated in broth of acoABCD mutants was converted to 2,3-butanediol. Parameters of R-acetoin production by budC mutants were optimized in batch culture. Aerobic culture and mildly acidic conditions (pH 6-6.5) favored R-acetoin accumulation. At the optimized conditions, in fed-batch fermentation, 62.3g/L R-acetoin was produced by budC and acoABCD double mutant in 57h culture, with an optical purity of 98.0%, and a substrate conversion ratio of 28.7%. Society for Industrial Microbiology and Biotechnology, 2015 R -acetoin nationallicence Acetoin dehydrogenase enzyme system nationallicence 2,3-Butanediol dehydrogenase nationallicence Klebsiella pneumoniae nationallicence Wang Dexin Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut Zhou Jidong Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut Chen Chuan Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut Wei Dong Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut Shi Jiping Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut Jiang Biao Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut Liu Pengfu Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut Hao Jian Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/8(2015-08-01), 1105-1115 1367-5435 42:8<1105 2015 42 10295 https://doi.org/10.1007/s10295-015-1638-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10295-015-1638-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Dexin Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Jidong Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Chen Chuan Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wei Dong Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Shi Jiping Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Biao Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Liu Pengfu Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Hao Jian Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, 201210, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Journal of Industrial Microbiology & Biotechnology Springer Berlin Heidelberg 42/8(2015-08-01), 1105-1115 1367-5435 42:8<1105 2015 42 10295